Viollier A F, Sabo G, Cardile N, Weschler D, Schmidlin C
Institut Dr. Viollier, Basel.
Schweiz Med Wochenschr. 1993 Jan 23;123(3):61-4.
A prospective study was performed on peripheral EDTA blood samples mailed for screening purposes to a hematology laboratory. Twenty-one samples which were suspicious for hairy cell leukemia (H 6000, Technicon Co. or STKS, Coulter Co.), were evaluated using three diagnostic tests: Microscopic morphology (Giemsa stain); Microscopic controlled inhibition of lymphocyte acid phosphatase by tartrate; Flow-cytometric immunophenotyping with monoclonal antibodies (Epics Profile, Coulter Co.). A characteristic immunophenotype was found on all typical hairy cell leukemias (except two) by positivity of CD11c, CD19, CD22, and CD25. The above-mentioned immunophenotype on peripheral lymphocytes, which can moreover be supported by demonstrating positivity for FMC7, is diagnostic for hairy cell leukemia. It therefore represents a rather straightforward tool to the bone-marrow diagnosis.
一项前瞻性研究对邮寄至血液学实验室用于筛查目的的外周乙二胺四乙酸（EDTA）血样进行。对21份疑似毛细胞白血病的样本（H 6000，Technicon公司或STKS，库尔特公司），使用三种诊断测试进行评估：显微镜形态学（吉姆萨染色）；酒石酸对淋巴细胞酸性磷酸酶的显微镜控制抑制；用单克隆抗体进行流式细胞术免疫表型分析（Epics Profile，库尔特公司）。除两例外，所有典型毛细胞白血病通过CD11c、CD19、CD22和CD25阳性呈现出特征性免疫表型。外周淋巴细胞上的上述免疫表型，此外还可通过证明FMC7阳性得到支持，对毛细胞白血病具有诊断意义。因此，它是骨髓诊断的一种相当直接的工具。
