Jaspar J M, Franchimont P
Eur J Cancer Clin Oncol. 1985 Nov;21(11):1343-8. doi: 10.1016/0277-5379(85)90315-3.
Epidermal growth factor (EGF) was assayed radioimmunologically in three secretions of the human mammary gland: milk (n = 16), colostrum (n = 8) and breast cyst fluid (n = 139). The immunoreactivity (immunoreactive EGF) detected in these three fluids corresponds directly to monomeric EGF, as can be shown by the complete cross-reaction with the standard and the identical behaviour in chromatography on Sephadex G50. The mean concentration of EGF is significantly greater in breast cyst fluid (241 +/- 143 ng/ml) and in colostrum (197 +/- 56 ng/ml) than in milk (107 +/- 22 ng/ml). The range of individual values in cyst fluid is large, 5-945 ng/ml. A possible role of EGF as a paracrine or autocrine factor in the pathology of cystic dysplasia of the mammary gland and in the increased risk of malignant transformation is hypothesised.
采用放射免疫法对人乳腺的三种分泌物进行了表皮生长因子（EGF）检测：乳汁（n = 16）、初乳（n = 8）和乳腺囊肿液（n = 139）。在这三种液体中检测到的免疫反应性（免疫反应性EGF）直接对应于单体EGF，这可通过与标准品的完全交叉反应以及在Sephadex G50上的色谱行为相同来证明。乳腺囊肿液（241±143 ng/ml）和初乳（197±56 ng/ml）中EGF的平均浓度显著高于乳汁（107±22 ng/ml）。囊肿液中个体值的范围很大，为5 - 945 ng/ml。推测EGF在乳腺囊性增生症的病理过程以及恶性转化风险增加中可能作为旁分泌或自分泌因子发挥作用。
