通过非放射性原位杂交技术对转谷氨酰胺酶1 mRNA在正常和银屑病表皮中的定位研究

Localization of transglutaminase 1 mRNA in normal and psoriatic epidermis by non-radioactive in situ hybridization.

作者信息

Nonomura K, Yamanishi K, Hosokawa Y, Doi H, Hirano J, Fukushima S, Yasuno H

机构信息

Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.

出版信息

Br J Dermatol. 1993 Jan;128(1):23-8. doi: 10.1111/j.1365-2133.1993.tb00141.x.

DOI:10.1111/j.1365-2133.1993.tb00141.x

PMID:7678985

Abstract

We examined the expression of the transglutaminase 1 (TGase 1) gene in frozen sections of normal and psoriatic epidermis by means of non-radioactive in situ hybridization with digoxigenin-labelled cRNA probes. TGase 1 mRNA was expressed in the granular layer of normal epidermis, regardless of ortho- or hyperkeratosis. However, in psoriatic epidermis, TGase 1 mRNA was detected in the suprabasal spinous layer, but not in the subcorneal layer. These results indicate that TGase 1 gene expression is limited to the last stage of keratinization in normal epidermis and this regulation is disturbed in psoriasis.

摘要

我们通过使用地高辛标记的cRNA探针进行非放射性原位杂交，检测了正常和银屑病表皮冰冻切片中转谷氨酰胺酶1（TGase 1）基因的表达。无论存在正角化还是角化过度，TGase 1 mRNA均在正常表皮的颗粒层中表达。然而，在银屑病表皮中，TGase 1 mRNA在基底层上方的棘层中被检测到，但在角质层下层未检测到。这些结果表明，TGase 1基因表达在正常表皮中仅限于角质化的最后阶段，而在银屑病中这种调节受到干扰。