Nonomura K, Yamanishi K, Yasuno H, Nara K, Hirose S
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
J Invest Dermatol. 1994 Jul;103(1):88-91. doi: 10.1111/1523-1747.ep12391802.
The expression of mRNA for elafin/SKALP, an inhibitor of leukocyte elastase and proteinase 3, in human normal and psoriatic epidermis was examined by in situ hybridization. In normal epidermis, elafin/SKALP mRNA was detected in the granular layer, but not in the spinous or basal layers. In fully developed psoriatic lesions, elafin/SKALP mRNA was found in the suprabasal layers of the ridges, and in the upper two thirds of the stratum malpighii at the elongated rete ridges. Intense staining was noted near the subcorneal microabscess in psoriasis vulgaris and under the subcorneal pustule in localized pustular psoriasis. In the marginal psoriatic epidermis, elafin/SKALP mRNA was expressed from the middle or upper spinous layer to the subcorneal layer, and the cells expressing elafin/SKALP mRNA increased especially under the parakeratotic corneal layer intermingled with pyknotic nuclei of neutrophils. These findings suggest that the induction of elafin/SKALP gene expression is related closely to the infiltration of neutrophils into the epidermis in psoriasis and plays an important role in protecting the skin components against the tissue damage caused by the infiltrated leukocytes.
通过原位杂交技术检测了白细胞弹性蛋白酶和蛋白酶3的抑制剂elafin/SKALP的mRNA在人正常及银屑病表皮中的表达情况。在正常表皮中，elafin/SKALP mRNA在颗粒层中被检测到，但在棘层或基底层中未检测到。在完全发展的银屑病皮损中，elafin/SKALP mRNA在嵴的基底层上方各层以及伸长的 rete 嵴处的马尔皮基层上三分之二中被发现。在寻常型银屑病的角质层下微脓肿附近以及局限性脓疱型银屑病的角质层下脓疱下方观察到强烈染色。在银屑病边缘表皮中，elafin/SKALP mRNA从棘层中部或上部至角质层下均有表达，且表达elafin/SKALP mRNA的细胞尤其在与嗜中性粒细胞固缩核混合的角化不全角质层下方增多。这些发现表明，elafin/SKALP基因表达的诱导与银屑病中嗜中性粒细胞向表皮的浸润密切相关，并在保护皮肤成分免受浸润白细胞引起的组织损伤中起重要作用。
