Voskuhl R R, McFarlin D E, Stone R, McFarland H F
Neuroimmunology Branch, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
J Neuroimmunol. 1993 Feb;42(2):187-91. doi: 10.1016/0165-5728(93)90009-n.
The major isoform of myelin basic protein (MBP) in the healthy adult central nervous system is the 18.5-kDa protein which is produced by mRNA derived from exons 1, 3, 4, 5, 6 and 7 of the MBP gene. Since isoforms containing exon 2-encoded protein (X2MBP) are expressed during myelin formation, we examined T cell reactivity specific for X2MBP in a disease characterized by remyelination subsequent to demyelination, multiple sclerosis (MS). T cell lines specific for X2MBP were derived from three MS patients as well as one healthy control. This suggests that candidate autoantigens in demyelinating/remyelinating diseases should include not only the major isoforms of myelin proteins, but also isoforms expressed aberrantly during a disease process since they too may be the target of a T cell-mediated autoimmune process.
在健康成人中枢神经系统中，髓鞘碱性蛋白（MBP）的主要异构体是18.5 kDa蛋白，它由源自MBP基因外显子1、3、4、5、6和7的mRNA产生。由于含外显子2编码蛋白的异构体（X2MBP）在髓鞘形成过程中表达，我们在一种以脱髓鞘后再髓鞘形成为特征的疾病——多发性硬化症（MS）中，检测了针对X2MBP的T细胞反应性。针对X2MBP的T细胞系来自三名MS患者以及一名健康对照。这表明，脱髓鞘/再髓鞘形成疾病中的候选自身抗原不仅应包括髓鞘蛋白的主要异构体，还应包括在疾病过程中异常表达的异构体，因为它们也可能是T细胞介导的自身免疫过程的靶点。
