Voskuhl R R, Martin R, McFarland H F
Neuroimmunology Branch, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
J Neuroimmunol. 1993 Feb;42(2):199-207. doi: 10.1016/0165-5728(93)90011-m.
This study has examined the cellular response to myelin basic protein (MBP) in a multiplex family with multiple sclerosis (MS). A total of 81 MBP-specific T cell lines (TCLs) were derived from three affected siblings and four healthy siblings. No difference was observed in estimated precursor frequencies of MBP-specific TCLs or peptide specificity of TCLs when comparing affected and unaffected siblings. MBP-specific TCLs from affected siblings, however, were restricted to the DRw15/DQw6 allele more frequently than those from unaffected siblings (P < 0.02). These data suggest that restriction of autoantigen-specific T cells may be the functional basis for disease susceptibility related to HLA class II inheritance.
本研究检测了一个患有多发性硬化症（MS）的多重家庭中细胞对髓鞘碱性蛋白（MBP）的反应。共从三名患病兄弟姐妹和四名健康兄弟姐妹中获得了81个MBP特异性T细胞系（TCL）。比较患病和未患病的兄弟姐妹时，未观察到MBP特异性TCL的估计前体频率或TCL的肽特异性存在差异。然而，与未患病兄弟姐妹的MBP特异性TCL相比，患病兄弟姐妹的MBP特异性TCL更频繁地受限 于DRw15/DQw6等位基因（P < 0.02）。这些数据表明，自身抗原特异性T细胞的受限可能是与HLA II类遗传相关的疾病易感性的功能基础。
