Márquez-Márquez R, Madrigal-Bujaidar E, Tejada de Hernández I
CENID Microbiología, Palo Alto Cuajimalpa, Mexico.
Mutat Res. 1993 Mar;299(1):1-8. doi: 10.1016/0165-1218(93)90112-q.
Aflatoxin B1 (AFB1) is a major contaminant in different agricultural products including maize. In an attempt to reduce this problem and the hazards to human health, an AFB1 inactivating system with ammonia has been developed. In this work we evaluated the efficiency of the system in mice using micronucleus (MN) and sister chromatid exchange (SCE) analysis. Four groups of animals were fed during 8 weeks with a special diet mainly composed of maize: (1) uncontaminated; (2) uncontaminated/inactivated; (3) contaminated/inactivated; and (4) contaminated. We evaluated MN at weekly intervals in peripheral blood, and in weeks 4 and 8 SCE frequencies were quantified in bone marrow cells. The results showed that animals fed with AFB1 contaminated/inactivated maize had a 45% lower level of induced cytogenetic damage than those animals fed with AFB1 contaminated, but not inactivated maize. A residual amount of AFB1 after the inactivating treatment and the reconversion back to AFB1 in the organism may account for the remaining increased levels of SCE and MN.
黄曲霉毒素B1（AFB1）是包括玉米在内的不同农产品中的主要污染物。为了减少这一问题以及对人类健康的危害，已开发出一种用氨使AFB1失活的系统。在这项工作中，我们使用微核（MN）和姐妹染色单体交换（SCE）分析评估了该系统在小鼠中的效率。四组动物连续8周喂食主要由玉米组成的特殊饮食：（1）未受污染的；（2）未受污染/已失活的；（3）受污染/已失活的；（4）受污染的。我们每周对外周血中的MN进行评估，并在第4周和第8周对骨髓细胞中的SCE频率进行定量。结果表明，喂食AFB1污染/已失活玉米的动物诱导的细胞遗传损伤水平比喂食AFB1污染但未失活玉米的动物低45%。失活处理后残留的AFB1量以及在生物体内重新转化为AFB1可能是SCE和MN水平仍然升高的原因。
