Madrigal-Santillán E, Madrigal-Bujaidar E, Márquez-Márquez R, Reyes A
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, Mexico.
Food Chem Toxicol. 2006 Dec;44(12):2058-63. doi: 10.1016/j.fct.2006.07.006. Epub 2006 Aug 7.
The potential of Saccharomyces cerevisiae (Sc) was evaluated for reducing the micronucleated normochromatic erythrocytes (MNNE) rate in mice fed AFB(1) contaminated corn. The study included two groups fed AFB(1) contaminated corn (0.4 and 0.8 mg/kg), a control fed uncontaminated corn, another group fed uncontaminated corn and 0.3% of Sc (1 x 10(8) live cells/g), and two groups fed AFB(1) contaminated corn (0.4 and 0.8 mg/kg) plus 0.3% Sc. Weight and MNNE were determined weekly for six weeks. Subsequently, the same determinations were made for another three-week period, but in mice receiving only a normal diet, without AFB(1) and Sc. Results in the first period revealed the following: control and Sc fed mice had similar constant weight increase, and low MNNE rate; mice fed only AFB(1) showed weight decrease and significant MNNE increase; finally, Sc improved weight gain and reduced MNNE produced by AFB(1). In the second period, results exhibited a tendency similar to that of the previous phase in the control and Sc fed mice; the weight and MNNE values improved in the other groups. We also determined the capacity of Sc for adsorbing and modifying the mycotoxin structure. The mixture was filtered to obtain two phases, and AFB(1) content was measured. Sc revealed a potent adsorbent capacity; however, chromatographic determination suggested no structural modification.
评估了酿酒酵母（Sc）对降低食用黄曲霉毒素B1（AFB1）污染玉米的小鼠微核正染红细胞（MNNE）率的潜力。该研究包括两组喂食AFB1污染玉米（0.4和0.8毫克/千克）的小鼠、一组喂食未污染玉米的对照小鼠、另一组喂食未污染玉米和0.3% Sc（1×10⁸个活细胞/克）的小鼠，以及两组喂食AFB1污染玉米（0.4和0.8毫克/千克）加0.3% Sc的小鼠。连续六周每周测定体重和MNNE。随后，在另外三周内对仅接受正常饮食、无AFB1和Sc的小鼠进行同样的测定。第一阶段的结果显示如下：对照小鼠和喂食Sc的小鼠体重持续增加情况相似，且MNNE率较低；仅喂食AFB1的小鼠体重下降且MNNE显著增加；最后，Sc改善了体重增加并降低了AFB1产生的MNNE。在第二阶段，对照小鼠和喂食Sc的小鼠的结果呈现出与前一阶段相似的趋势；其他组的体重和MNNE值有所改善。我们还测定了Sc吸附和改变霉菌毒素结构的能力。将混合物过滤以获得两相，并测量AFB1含量。Sc显示出强大的吸附能力；然而，色谱测定表明其结构未发生改变。
