利用体外表达和放射免疫沉淀法评估传染性法氏囊病病毒的VP2表位
Evaluation of VP2 epitopes of infectious bursal disease virus using in vitro expression and radioimmunoprecipitation.
作者信息
Crisman J M, Jackwood R J, Lana D P, Jackwood D J
机构信息
Department of Veterinary Preventive Medicine, Ohio State University, Wooster.
出版信息
Arch Virol. 1993;128(3-4):333-44. doi: 10.1007/BF01309443.
A clone (pV 17-7) spanning a portion of the VP2 gene of infectious bursal disease virus (IBDV) was selected from a cDNA library produced using the variant A virus strain. This clone was expressed in vitro and the protein products were immunoprecipitated with various virus-neutralizing antisera made against 6 different strains of IBDV. The antisera made against 4 variant strains immunoprecipitated the translation products from the pV 17-7 clone, but the antisera to the classic STC virus and the serotype 2 OH virus did not immunoprecipitate the pV 17-7 translation products.
从使用变异A病毒株构建的cDNA文库中筛选出一个跨越传染性法氏囊病病毒（IBDV）VP2基因部分区域的克隆（pV 17-7）。该克隆在体外表达，其蛋白产物用针对6种不同IBDV毒株制备的各种病毒中和抗血清进行免疫沉淀。针对4种变异毒株制备的抗血清能免疫沉淀pV 17-7克隆的翻译产物，但针对经典STC病毒和2型OH病毒的抗血清不能免疫沉淀pV 17-7的翻译产物。
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