Bayliss C D, Peters R W, Cook J K, Reece R L, Howes K, Binns M M, Boursnell M E
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, U.K.
Arch Virol. 1991;120(3-4):193-205. doi: 10.1007/BF01310475.
The coding sequences of VP2 from a virulent strain, 52/70, of infectious bursal disease virus (IBDV) were excised from a cDNA clone and inserted into a fowlpox plasmid insertion vector. The resulting plasmid, pIBD 1, was used to construct a recombinant fowlpox virus, fpIBD 1, which expressed VP 2 as a beta-galactosidase fusion protein. Chickens vaccinated with fpIBD 1 at 1 and 14 days of age, were challenged at 28 days with either IBDV strain 52/70 or the highly virulent strain CS 89. These chickens were protected against mortality, but not against damage to the bursa of Fabricius. The protection achieved by the use of fpIBD 1 shows that VP 2 is a host protective antigen.
从传染性法氏囊病病毒（IBDV）强毒株52/70的cDNA克隆中切下VP2的编码序列，并将其插入禽痘病毒质粒插入载体中。由此得到的质粒pIBD 1用于构建重组禽痘病毒fpIBD 1，该病毒将VP2表达为β-半乳糖苷酶融合蛋白。在1日龄和14日龄用fpIBD 1免疫的鸡，在28日龄时用IBDV毒株52/70或高毒株CS 89进行攻毒。这些鸡可免受死亡，但不能防止法氏囊受到损伤。使用fpIBD 1所获得的保护表明VP2是一种宿主保护性抗原。
