Haber R, Bessette D, Hulihan-Giblin B, Durcan M J, Goldman D
Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892.
J Neurochem. 1993 Mar;60(3):1159-62. doi: 10.1111/j.1471-4159.1993.tb03269.x.
The gene for tryptophan 2,3-dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA-PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain.
迄今为止，人们认为色氨酸2,3-双加氧酶（TDO）基因仅在肝脏中表达。此处给出的数据表明，编码TDO的RNA存在于啮齿动物的大脑中。基于大鼠肝脏TDO cDNA序列合成了寡核苷酸引物，并通过RNA-PCR用于扩增源自小鼠全脑、肝脏以及大鼠脑区的RNA。反应产物经过纯化后进行DNA测序。当扩增小鼠全脑和肝脏RNA时获得了相同的序列，并且这些序列显示与已发表的大鼠肝脏色氨酸TDO cDNA序列有96%的同一性。此外，在源自大鼠脑干、小脑、皮质、下丘脑以及大脑其余部分的RNA中也发现了TDO序列。
