Murphy P D, Wadhera V, Griffin S M, Burgess P, Farrell D, Taylor I, Hair T, Clague M B, Griffith C D
Department of Surgery, Newcastle General Hospital, Newcastle upon Tyne, UK.
J R Coll Surg Edinb. 1993 Feb;38(1):28-32.
The finding of free tumour cells within the peritoneum at the time of laparotomy for gastrointestinal cancer is an important indicator of prognosis and may help select treatment. The aim of this study was to improve the methods whereby these cells could be retrieved and identified. Free peritoneal cancer cells were found in 6 out of 18 gastric cancer patients. All 6 patients had tumour invasion through to the serosa and subsequently died of tumour recurrence. None of the 18 Dukes' B and C colorectal cancer patients had free peritoneal cancer cells. Only 2 of a further 5 patients with extensive colorectal tumour spread had positive cytologies. The conventional mucin stain Periodic Acid Schiff (after diastase digestion) and the monoclonal antibody stain B72.3 were the most useful in identifying free peritoneal cancer cells. Peritoneal brushings did not offer any advantages over conventional peritoneal washings in the retrieval of free tumour cells.
在因胃肠道癌行剖腹手术时，在腹膜内发现游离肿瘤细胞是预后的一个重要指标，且可能有助于选择治疗方案。本研究的目的是改进这些细胞的获取和鉴定方法。18例胃癌患者中有6例发现了游离的腹膜癌细胞。所有6例患者的肿瘤均侵犯至浆膜层，随后均死于肿瘤复发。18例Dukes B期和C期结直肠癌患者均未发现游离的腹膜癌细胞。另外5例结直肠癌广泛转移患者中只有2例细胞学检查呈阳性。传统的黏液染色高碘酸希夫染色法（淀粉酶消化后）和单克隆抗体染色B72.3在鉴定游离腹膜癌细胞方面最为有用。在获取游离肿瘤细胞方面，腹膜刷检与传统的腹膜冲洗相比没有任何优势。
