日光性角化病中肿瘤抑制基因p53产物和嘧啶二聚体的免疫组织化学检查

Immunohistochemical examination of tumor-suppressor gene p53 product and pyrimidine dimer in solar keratosis.

作者信息

Taguchi M, Watanabe S, Sato Y, Kameya T, Munakata N, Ishihara K, Nakane P K, Hisatome H, Ikeda S

机构信息

Epidemiology Division, National Cancer Center Research Institute, Tokyo, Japan.

出版信息

J Cancer Res Clin Oncol. 1993;119(5):260-2. doi: 10.1007/BF01212722.

DOI:10.1007/BF01212722

PMID:7680043

Abstract

In order to find biomarkers to measure the effects of UV irradiation, we examined the accumulation of p53 protein and pyrimidine dimers in 18 solar keratosis specimens. Frozen or AMeX-fixed solar keratosis specimens were immunohistochemically stained by anti-p53 mouse monoclonal antibody, pAb1801 and polyclonal anti-(pyrimidine dimer) antibody. Nuclear accumulation of p53 protein was found in 5/18 (28%) solar keratosis lesions. The percentage of cases showing nuclear p53 protein varied according to the histological type; in the bowenoid type it was 4/7 (57%); in the atrophic type it was 1/7 (14%). Nuclear pyrimidine dimers were not stained in solar keratosis, although the skin of UV-irradiated nude mice was positive. Accumulation of p53 protein is a good marker for early precancerous change caused by UV exposure.

摘要

为了寻找能够测量紫外线辐射影响的生物标志物，我们检测了18个日光性角化病标本中p53蛋白和嘧啶二聚体的积累情况。冷冻或AmeX固定的日光性角化病标本用抗p53小鼠单克隆抗体pAb1801和多克隆抗（嘧啶二聚体）抗体进行免疫组织化学染色。在18个日光性角化病病变中有5个（28%）发现p53蛋白在细胞核中积累。显示细胞核p53蛋白的病例百分比因组织学类型而异；在鲍温样型中为4/7（57%）；在萎缩型中为1/7（14%）。尽管紫外线照射的裸鼠皮肤呈阳性，但日光性角化病中细胞核嘧啶二聚体未被染色。p53蛋白的积累是紫外线暴露引起的早期癌前变化的良好标志物。