Mondain M, Ryan A
Department of Surgery/Otolaryngology, University of California, San Diego School of Medicine.
Laryngoscope. 1993 Mar;103(3):312-8. doi: 10.1288/00005537-199303000-00012.
Basic fibroblast growth factor (bFGF) can stimulate the proliferation and differentiation of keratinocytes, fibroblasts, and endothelial cells. These cells are involved during the healing of tympanic membrane (TM) perforations. Light and electron microscopy examinations were used to study the histology of TM healing after application of 400 ng of bFGF on the perforation. The progress of healing is accelerated, but the basic healing process is unchanged, i.e., epithelial proliferation first closes the perforation and is then followed by connective tissue growth. There is more connective tissue in the TM receiving bFGF, and extracellular fibers are better oriented. No significant increase of neoangiogenesis was detected in the treated TM. In the nonperforated area of treated TM, an extensive hyperplasia of the submucosal connective tissue is observed. These results demonstrate that bFGF can produce a TM scar containing more connective tissue, which may be of benefit in the prevention of atrophic healed TM.
碱性成纤维细胞生长因子（bFGF）可刺激角质形成细胞、成纤维细胞和内皮细胞的增殖与分化。这些细胞参与鼓膜（TM）穿孔的愈合过程。运用光学显微镜和电子显微镜检查来研究在穿孔处应用400 ng bFGF后TM愈合的组织学情况。愈合进程加快，但基本愈合过程未变，即上皮细胞增殖首先封闭穿孔，随后是结缔组织生长。接受bFGF的TM中有更多结缔组织，细胞外纤维排列更规整。在接受治疗的TM中未检测到新生血管形成显著增加。在接受治疗的TM的非穿孔区域，观察到黏膜下结缔组织广泛增生。这些结果表明，bFGF可产生含有更多结缔组织的TM瘢痕，这可能有助于预防愈合后的TM萎缩。
