Seo K Y, Riley J, Cortez D, Wagner E D, Plewa M J
Institute for Environmental Studies, University of Illinois, Urbana-Champaign 61801.
Mutat Res. 1993 Apr;299(2):111-20. doi: 10.1016/0165-1218(93)90088-u.
Monocyclic aromatic amines are environmental contaminants and many are promutagens and procarcinogens. Cultured tobacco cells, strain TX1, activated m-phenylenediamine into a frameshift mutagen that reverted the hisD3052 allele in Salmonella typhimurium strains TA98 and YG1024. However, the plant-activated products were refractory in strain TA98/1,8-DNP6. This indicated that these plant-activated products were substrates for bacterial acetyl-CoA: N-hydroxyarylamine O-acetyltransferase. A stable, high molecular weight (> 300 kDa) proximal mutagen was isolated by molecular ultrafiltration membranes. No parent compound was associated with the isolated mutagenic fraction. The high molecular weight fraction induced mutation in S. typhimurium strains TA98, YG1021 and YG1024. From these data we propose a model for the plant-activation of aromatic amine promutagens.
单环芳香胺是环境污染物，其中许多是前诱变剂和前致癌物。培养的烟草细胞系TX1将间苯二胺激活为一种移码诱变剂，该诱变剂可使鼠伤寒沙门氏菌TA98和YG1024菌株中的hisD3052等位基因回复突变。然而，植物激活产物在TA98/1,8-DNP6菌株中无活性。这表明这些植物激活产物是细菌乙酰辅酶A：N-羟基芳胺O-乙酰基转移酶的底物。通过分子超滤膜分离出一种稳定的、高分子量（>300 kDa）的近端诱变剂。分离出的诱变部分未与母体化合物相关联。高分子量部分在鼠伤寒沙门氏菌TA98、YG1021和YG1024菌株中诱导突变。根据这些数据，我们提出了一个芳香胺前诱变剂植物激活的模型。
