Trebichavský I, Nyklícek O
Institute of Microbiology, Department of Immunology and Gnotobiology, Prague.
Contraception. 1993 Feb;47(2):205-14. doi: 10.1016/0010-7824(93)90092-l.
The nature of adherent cells obtained from the surface of intrauterine contraceptive devices (IUD) was investigated by immunofluorescence using antibodies directed against tumor necrosis factor alpha (TNF), leukocyte differentiation antigens, immunoglobulin isotypes and J chain. Macrophages/monocytes, lymphocytes and neutrophils were found in all fifty samples. Macrophages were characterized by CD14, CD15 and CD71 positivity, DAKO macrophage antibody and HLA-DR, CD11c and CD45 expression. B cells contained secretory IgA with J chain or IgG and some cells expressed surface IgM. Neither IgD nor IgE were found. T-antigens CD3, CD4 and CD5 were not detected, the CD8 was faintly expressed. The CD56 molecule identifying NK cells was found on small lymphocytes. Cytoplasmic C3 protein was detected in neutrophils. Tumor necrosis factor alpha (TNF) was visualized in 14.3 +/- 8.8% of nucleated IUD cells. This cytokine was localized in the cytoplasm of the macrophages and the lymphocytes.
通过使用针对肿瘤坏死因子α（TNF）、白细胞分化抗原、免疫球蛋白同种型和J链的抗体进行免疫荧光，研究了从宫内节育器（IUD）表面获得的贴壁细胞的性质。在所有五十个样本中均发现了巨噬细胞/单核细胞、淋巴细胞和中性粒细胞。巨噬细胞的特征是CD14、CD15和CD71呈阳性，DAKO巨噬细胞抗体以及HLA-DR、CD11c和CD45表达。B细胞含有带有J链的分泌型IgA或IgG，一些细胞表达表面IgM。未发现IgD和IgE。未检测到T抗原CD3、CD4和CD5，CD8微弱表达。在小淋巴细胞上发现了识别NK细胞的CD56分子。在中性粒细胞中检测到细胞质C3蛋白。在14.3±8.8%的有核IUD细胞中可见肿瘤坏死因子α（TNF）。这种细胞因子定位于巨噬细胞和淋巴细胞的细胞质中。
