Zhuo H, Helke C J
Department of Pharmacology, Uniformed Services University of the Health Science, Bethesda, MD 20814-4799.
Neuroscience. 1993 Feb;52(4):1019-28. doi: 10.1016/0306-4522(93)90548-t.
The presence and location of CNS neurokinin B peptide-2-like immunoreactive neurons that project to the spinal cord were studied by a combination of retrograde transport of fluorescent dye (FluoroGold) and fluorescence immunocytochemistry. After injections of FluoroGold into the thoracic or lumbar segments of the rat spinal cord, serial sections of brain were stained with antisera directed against neurokinin B peptide-2. The results of the study showed that neurokinin B peptide-2-like immunoreactive neurons were located in the nucleus arcuate, median eminence, ventral and external bed nuclei of the stria terminalis, dorsal hypothalamic area, and medial habenula. Neurokinin B peptide-2 neurons that give rise to the long descending projections from the hypothalamus to thoracolumbar spinal cord were found only in the dorsal hypothalamic area. Approximately 36% of the neurokinin B peptide-2 neurons in the dorsal hypothalamic area projected to the spinal cord, whereas about 28% of the spinal projecting neurons in the dorsal hypothalamic area contained neurokinin B peptide-2-like immunoreactivity. Most of the spinal projecting neurokinin B peptide-2 neurons in the dorsal hypothalamic area had a cell size of 15 x 25 microns. In the spinal cord, immunoreactive neurokinin B peptide-2 fibers and terminals were distributed mainly in the superficial dorsal horn and the central autonomic area, with the highest density in laminae II and X, with less density in laminae IV and V. A few neurokinin peptide-2 fibers and terminals were also found in the ventral horn of the spinal cord. The results of the present study show that hypothalamic neurokinin B peptide-2 neurons are the main source of the spinal neurokinin B peptide-2.
采用荧光染料（FluoroGold）逆行运输和荧光免疫细胞化学相结合的方法，研究了投射至脊髓的中枢神经系统神经激肽B肽 - 2样免疫反应性神经元的存在及位置。将FluoroGold注入大鼠脊髓胸段或腰段后，用抗神经激肽B肽 - 2的抗血清对脑的连续切片进行染色。研究结果表明，神经激肽B肽 - 2样免疫反应性神经元位于弓状核、正中隆起、终纹床核腹侧和外侧、下丘脑背侧区以及内侧缰核。仅在下丘脑背侧区发现了从中枢下丘脑向胸腰段脊髓发出长下行投射的神经激肽B肽 - 2神经元。下丘脑背侧区约36%的神经激肽B肽 - 2神经元投射至脊髓，而下丘脑背侧区约28%的投射至脊髓的神经元含有神经激肽B肽 - 2样免疫反应性。下丘脑背侧区大多数投射至脊髓的神经激肽B肽 - 2神经元的细胞大小为15×25微米。在脊髓中，免疫反应性神经激肽B肽 - 2纤维和终末主要分布在背角浅层和中枢自主区，在Ⅱ层和Ⅹ层密度最高，在Ⅳ层和Ⅴ层密度较低。在脊髓腹角也发现了少量神经激肽肽 - 2纤维和终末。本研究结果表明，下丘脑神经激肽B肽 - 2神经元是脊髓神经激肽B肽 - 2的主要来源。
