Cherif D, Romana S, Der-Sarkissian H, Jones C, Berger R
INSERM U 301, Institut de Génétique Moléculaire, Paris, France.
Genes Chromosomes Cancer. 1993 Feb;6(2):107-12. doi: 10.1002/gcc.2870060207.
An Alu polymerase chain reaction (PCR) probe specific for chromosome 11 prepared from the somatic cell hybrid J1 was used to analyze karyotypes of eight patients with acute monocytic leukemia (AML-M5). Chromosome painting confirmed the t(9;11) in one patient and a der(1)t(1;6)t(6;11) in another and allowed the identification of a complex rearrangement involving chromosomes 9, 11, and 17, previously classified as del(11)(q23), in a third patient. An analysis of five patients with AML-M5 and a normal karyotype did not detect abnormalities of chromosome 11. The usefulness of chromosome painting combined with in situ hybridization with probes previously located on particular chromosomes is emphasized.
使用从体细胞杂种J1制备的针对11号染色体的Alu聚合酶链反应（PCR）探针，分析了8例急性单核细胞白血病（AML-M5）患者的核型。染色体描绘证实1例患者存在t(9;11)，另1例患者存在der(1)t(1;6)t(6;11)，并使第3例患者中涉及9号、11号和17号染色体的复杂重排得以鉴定，该重排先前被归类为del(11)(q23)。对5例核型正常的AML-M5患者进行分析，未检测到11号染色体异常。强调了染色体描绘与先前定位在特定染色体上的探针进行原位杂交相结合的实用性。
