Gorospe W C, Spangelo B L
Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston 29425.
Biol Reprod. 1993 Mar;48(3):538-43. doi: 10.1095/biolreprod48.3.538.
In the present study we examined the influence of FSH as well as a number of well-established cytokines on interleukin (IL)-6 by rat granulosa cells in culture. Increasing concentrations of FSH, IL-1 alpha, IL-1 beta, tumor necrosis factor alpha (TNF alpha), and lipopolysaccharide (LPS) were incubated for 48 h with undifferentiated granulosa cells obtained from diethylstilbestrol-primed immature rats. The results demonstrate that FSH, IL-1 alpha, IL-1 beta, and LPS, but not TNF alpha, caused significant concentration-dependent increases in IL-6 release. We also examined the effects of dibutyryl-cAMP, forskolin, and 3-isobutyl-1-methyl-xanthine (IBMX) on IL-6 release by granulosa cells. Each of these agents caused a significant concentration-dependent increase in IL-6 production by granulosa cells in either the absence or presence of FSH. Taken together, these results show that the granulosa cell is not only a likely source of IL-6 but that the release of IL-6 can be regulated. Moreover, evidence suggests that cAMP may serve as a second messenger for the stimulated secretion of IL-6 by undifferentiated granulosa cells.
在本研究中，我们检测了促卵泡激素（FSH）以及一些成熟的细胞因子对培养的大鼠颗粒细胞白细胞介素（IL）-6的影响。将浓度递增的FSH、白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNFα）和脂多糖（LPS）与从己烯雌酚预处理的未成熟大鼠获得的未分化颗粒细胞一起孵育48小时。结果表明，FSH、IL-1α、IL-1β和LPS可引起IL-6释放呈显著的浓度依赖性增加，而TNFα则无此作用。我们还检测了二丁酰环磷腺苷（dibutyryl-cAMP）、福斯可林（forskolin）和3-异丁基-1-甲基黄嘌呤（IBMX）对颗粒细胞IL-6释放的影响。在不存在或存在FSH的情况下，这些试剂中的每一种都能使颗粒细胞IL-6的产生呈显著的浓度依赖性增加。综上所述，这些结果表明颗粒细胞不仅可能是IL-6的来源，而且IL-6的释放是可以调节的。此外，有证据表明，环磷腺苷（cAMP）可能作为未分化颗粒细胞刺激分泌IL-6的第二信使。
