Sirinathsinghji D J, Mayer E, Stam R, Fernandez J M, Dunnett S B
Department of Neurobiology, AFRC Institute of Animal Physiology and Genetics Research, Brabraham, Cambridge, UK.
Neuroreport. 1993 Feb;4(2):175-8. doi: 10.1097/00001756-199302000-00015.
We have studied by quantitative in situ hybridization histochemistry the mRNA expression of the growth associated phosphoprotein (GAP-43) in grafts of foetal striatal neurones implanted into the ibotenic acid lesioned adult rat neostriatum. Animals were studied at 7, 15, 30 and 90 days after implantation. Using a 35S-labelled specific oligonucleotide probe we observed the highest levels of GAP-43 mRNA in 15-day-old grafts; levels were significantly reduced at 30 days and in 3-month-old grafts GAP-43 mRNA expression was not significantly different from the very low levels in the normal rat striatum. The data indicate that GAP-43 mRNA is highly expressed in developing but not mature neurones and suggest that embryonic striatal grafts may develop a relatively adult phenotype within the host brain by about 4 weeks; by which time most of the synaptic connections are established.
我们通过定量原位杂交组织化学方法，研究了植入鹅膏蕈氨酸损伤的成年大鼠新纹状体中的胎儿纹状体神经元移植物中生长相关磷蛋白（GAP - 43）的mRNA表达。在植入后7天、15天、30天和90天对动物进行研究。使用35S标记的特异性寡核苷酸探针，我们观察到在15日龄的移植物中GAP - 43 mRNA水平最高；30天时水平显著降低，在3月龄移植物中，GAP - 43 mRNA表达与正常大鼠纹状体中的极低水平无显著差异。数据表明，GAP - 43 mRNA在发育中的而非成熟的神经元中高表达，提示胚胎纹状体移植物可能在约4周内在宿主脑内发育出相对成熟的表型；到那时大部分突触连接已经建立。
