Sirinathsinghji D J, Mayer E, Fernandez J M, Dunnett S B
Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK.
Neuroreport. 1993 Jun;4(6):659-62. doi: 10.1097/00001756-199306000-00014.
We have studied by in situ hybridization histochemistry the mRNA expression of preprocholecystokinin (CCK) in grafts of foetal striatal tissue neurones implanted into the ibotenic acid lesioned adult rat neostriatum. Animals were studied at 30 and 90 days after implantation. Using a 35S-labelled 'antisense' oligonucleotide (45-mer) probe complementary to the rat CCK cDNA we observed a dense hybridization signal for CCK mRNA in the striatal eminence in developing embryos and in all the striatal grafts examined; no CCK mRNA expressing cells were found in the normal neonatal or adult rat neostriatum. However, CCK mRNA was heavily expressed in other areas of the host brain including the somatosensory cortex, the claustrum, primary olfactory cortex and endopiriform cortex. The germinal cell layer of the striatal eminence is the source of origin for these non-striatal cell groups as well as for cells destined to form the neostriatum. The present results suggest that CCK mRNA expression provides an unambiguous marker for many of the non-striatal cells in 'striatal' grafts.
我们通过原位杂交组织化学方法研究了植入鹅膏蕈氨酸损伤的成年大鼠新纹状体的胎儿纹状体组织神经元移植物中前胆囊收缩素（CCK）mRNA的表达。在植入后30天和90天对动物进行研究。使用与大鼠CCK cDNA互补的35S标记的“反义”寡核苷酸（45聚体）探针，我们在发育中的胚胎纹状体隆起以及所有检查的纹状体移植物中观察到CCK mRNA的密集杂交信号；在正常新生或成年大鼠新纹状体中未发现表达CCK mRNA的细胞。然而，CCK mRNA在宿主脑的其他区域大量表达，包括体感皮层、屏状核、初级嗅觉皮层和内梨状皮层。纹状体隆起的生发细胞层是这些非纹状体细胞群以及注定形成新纹状体的细胞的起源。目前的结果表明，CCK mRNA表达为“纹状体”移植物中的许多非纹状体细胞提供了明确的标记。
