Böttger V, Micheel B, Scharte G, Kaiser G, Wolf G, Schmechta H
Max Delbrück Centre of Molecular Medicine, Berlin-Buch, Germany.
Hybridoma. 1993 Feb;12(1):81-91. doi: 10.1089/hyb.1993.12.81.
A panel of mouse monoclonal antibodies (MABs) was produced against human chorionic gonadotropin (HCG) and its isolated beta-subunit (beta-HCG). According to their binding specificities the antibodies could be divided into HCG-specific and cross-reactive MABs. The HCG-specific antibodies reacted with antigenic sites on holo-HCG or holo-HCG and beta-HCG, or exclusively with the non-associated beta-HCG chain. The cross-reactive antibodies reacted with either HCG and luteinizing hormone (LH) or with HCG, LH, follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH). According to the binding specificities of the MABs and their reciprocal inhibition detected in two-site binding enzyme immunoassays (EIA), altogether 13 epitopes (including the 3 hidden epitopes detectable only on free non-associated beta-HCG) were distinguished by the antibodies described here. Antibody combinations resulting in most effective and specific HCG- or beta-HCG-determination were used as clinical assays and proved their reliability and correctness for monitoring patients with HCG- and/or beta-HCG-producing tumors before and after therapy.
制备了一组小鼠单克隆抗体（MABs），用于针对人绒毛膜促性腺激素（HCG）及其分离的β亚基（β-HCG）。根据其结合特异性，这些抗体可分为HCG特异性和交叉反应性MABs。HCG特异性抗体与全人绒毛膜促性腺激素（holo-HCG）或全人绒毛膜促性腺激素以及β-HCG上的抗原位点反应，或仅与非结合的β-HCG链反应。交叉反应性抗体与HCG和促黄体生成素（LH）反应，或与HCG、LH、促卵泡生成素（FSH）和促甲状腺激素（TSH）反应。根据MABs的结合特异性及其在双位点结合酶免疫测定（EIA）中检测到的相互抑制作用，本文所述的抗体共区分出13个表位（包括仅在游离非结合β-HCG上可检测到的3个隐蔽表位）。产生最有效和特异性HCG或β-HCG测定的抗体组合被用作临床检测，并证明了它们在监测患有产生HCG和/或β-HCG肿瘤的患者治疗前后的可靠性和正确性。
