抗IgE和伴刀豆球蛋白A诱导的嗜碱性粒细胞组胺释放。与血浆总IgE含量的关系。

Basophil histamine release induced by anti-IgE and concanavalin A. Relation to the total plasma IgE content.

作者信息

Windelborg Nielsen B, Hansen B, Damsgaard T M, Herlin T, Søderberg U, Bjerke T, Thestrup-Pedersen K, Schiøtz P O

机构信息

Department of Pediatrics, University Hospital of Aarhus, Denmark.

出版信息

Allergy. 1993 Jan;48(1):54-61. doi: 10.1111/j.1398-9995.1993.tb02175.x.

DOI:10.1111/j.1398-9995.1993.tb02175.x

PMID:7681267

Abstract

The plant lectin concanavalin A (Con A) has been shown to induce basophil histamine release by an IgE-receptor-dependent process resembling that of anti-IgE-antibodies. In this study, the concentration-response for basophil histamine release from washed blood cells was analyzed in a population of blood samples from adults with a total plasma IgE content ranging from < 5 kU/l to > 18,000 kU/l (n = 108), as well as 148 cord blood samples. The concentration-response-curves for anti-IgE in all adult blood samples were similar, despite the large variation in total plasma IgE--only the cord blood samples showed a decreased sensitivity. In contrast, the optimal concentration of Con A was inversely related to plasma IgE, and this relation was most pronounced in the adult blood samples. It is proposed that IgE-receptor-mediated histamine release may be dependent not only on the number of stimulatory, dimeric cross-links formed between IgE-receptors, but also on the molecular structure of the cross-linking agent.

摘要

植物凝集素伴刀豆球蛋白A（Con A）已被证明可通过类似于抗IgE抗体的IgE受体依赖性过程诱导嗜碱性粒细胞释放组胺。在本研究中，分析了来自成人的108份血浆总IgE含量范围从<5 kU/l至>18,000 kU/l的血样以及148份脐带血样中，洗涤后的血细胞释放嗜碱性粒细胞组胺的浓度-反应情况。尽管血浆总IgE存在很大差异，但所有成人血样中抗IgE的浓度-反应曲线相似——只有脐带血样显示出敏感性降低。相比之下，Con A的最佳浓度与血浆IgE呈负相关，且这种关系在成人血样中最为明显。有人提出，IgE受体介导的组胺释放可能不仅取决于IgE受体之间形成的刺激性二聚体交联的数量，还取决于交联剂的分子结构。