Austin G, Fisher S, Dickson D, Anderson D, Richardson S
Aneurysm Research Laboratory, University of California, Santa Barbara.
Ann Clin Lab Sci. 1993 Mar-Apr;23(2):97-105.
To what extent might change sin the extracellular matrix proteins in intracranial aneurysms (IA) or parent artery (PA) wall contribute to enlargement? Using IA specimens obtained from the coroner's office, the wall structure by light (Trichrome and Actin stains) and immunofluorescent microscopy (IF) (collagen I and fibronectin) have been studied in 18 specimens. It is well known that IA only arise when there is a deficiency in the internal elastic membrane and some breakdown in the media. The results show a normal three layer structure of intima, media, (muscularis), and adventitia in the PA extending out into the neck of the aneurysm. Immunofluorescent stains (IS) show collagen I and fibronectin limited to the adventitia and muscularis, respectively, of the PA and control arteries. The enlarging IA wall shows almost complete loss of this architecture. By IF stain, collagen I and fibronectin are intermingled throughout the IA wall. It is postulated that loss of structure and the confining muscularis layer permit easier elastic enlargement from a more or less homogeneous wall structure.
颅内动脉瘤（IA）或载瘤动脉（PA）壁细胞外基质蛋白的变化在多大程度上会导致动脉瘤扩大？利用从验尸官办公室获取的IA标本，对18个标本进行了光镜（三色染色和肌动蛋白染色）和免疫荧光显微镜（IF）（I型胶原蛋白和纤连蛋白）观察以研究其壁结构。众所周知，只有当内弹性膜存在缺陷且中膜出现一些破坏时才会发生IA。结果显示，PA延伸至动脉瘤颈部处的内膜、中膜（肌层）和外膜具有正常的三层结构。免疫荧光染色（IS）显示，I型胶原蛋白和纤连蛋白分别局限于PA和对照动脉的外膜和肌层。扩大的IA壁几乎完全失去了这种结构。通过IF染色，I型胶原蛋白和纤连蛋白在整个IA壁中相互交织。据推测，结构的丧失和限制的肌层使得从或多或少均匀的壁结构更容易发生弹性扩张。
