Wang Y Z, Jia X A, Zhao J Y, Xu G L
Department of Biochemistry, Xi'an Medical University, Shaanxi, People's Republic of China.
Biol Trace Elem Res. 1993 Feb;36(2):159-66. doi: 10.1007/BF02783175.
Two groups of weanling Sprague-Dawley rats were fed a low-selenium basal diet (Se 0.009 mg/kg) and the same diet supplemented with sodium selenite (Se 0.25 mg/kg), respectively, for 1, 2, and 3 months. At each feeding time, the Ca(2+)-ATPase activity, Ca2+ uptake rate and the capacity of Ca2+ uptake in isolated cardiac sacroplasmic reticulum from the Se-deficient rats were decreased significantly compared to those from the Se-supplemented rats, the contents of lipid peroxide in postmitochondrial supernatant and isolated sarcoplasmic reticulum from the Se-deficient rats were significantly higher than that from Se-supplemented rats. Compared to the Se-supplemented rats, the cytosolic glutathione peroxidase activity in Se-deficient rats decreased significantly. In addition, significant linear negative correlations of lipid peroxide in postmitochondrial supernatant to sarcoplasmic reticular Ca(2+)-ATPase activity, Ca2+ uptake rate and to whole blood selenium concentration were observed. The results suggest that the enhancement of lipid peroxidation via the depressed glutathione peroxidase activity might be responsible for the decrease of Ca(2+)-ATPase and Ca2+ uptake activities in sarcoplasmic reticulum in Se-deficient animals.
将两组断乳的斯普拉格-道利大鼠分别喂食低硒基础日粮（硒含量0.009毫克/千克）和添加亚硒酸钠的相同日粮（硒含量0.25毫克/千克），持续1、2和3个月。在每个喂食时间点，与补充硒的大鼠相比，缺硒大鼠离体心肌肌浆网中的Ca(2+)-ATP酶活性、Ca2+摄取率和Ca2+摄取能力显著降低，缺硒大鼠线粒体后上清液和离体肌浆网中脂质过氧化物的含量显著高于补充硒的大鼠。与补充硒的大鼠相比，缺硒大鼠胞质谷胱甘肽过氧化物酶活性显著降低。此外，观察到线粒体后上清液中脂质过氧化物与肌浆网Ca(2+)-ATP酶活性、Ca2+摄取率以及全血硒浓度之间存在显著的线性负相关。结果表明，通过降低谷胱甘肽过氧化物酶活性增强脂质过氧化作用可能是缺硒动物肌浆网中Ca(2+)-ATP酶和Ca2+摄取活性降低的原因。
