Geffard M, Boullerne A, Brochet B
Laboratoire d'Immunologie et de Pathologie, Université de Bordeaux II, France.
Brain Res Bull. 1993;30(3-4):365-8. doi: 10.1016/0361-9230(93)90266-e.
Immune complexes from sera of MS patients, other neurological diseases, and healthy donors were precipitated using polyethyleneglycol and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Silver staining evidenced additional protein bands whose molecular weights were 14-16, 38, and 43 kDa. These IC proteins were present in most MS patients studied. To identify their nature, immunoblotting was performed with antihuman immunoglobulins A, M, G antibodies. No immunoreactivity was found below a molecular weight of 66 kDa on a nitrocellulose sheet having the transferred protein pattern of MS IC. Using purified human myelin, MS IC transferred to an immobilon sheet and antihuman myelin basic protein antibodies, an immunoreactivity was seen only on purified human MBP. The small proteins of 14-16 kDa and the others of 38, 43 kDa were not immunoreactive. Identification of the nature of these additional proteins in MS IC is in progress.
使用聚乙二醇沉淀多发性硬化症（MS）患者、其他神经系统疾病患者以及健康供体血清中的免疫复合物，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。银染显示出分子量为14 - 16 kDa、38 kDa和43 kDa的额外蛋白条带。这些免疫复合物蛋白存在于大多数所研究的MS患者中。为了确定它们的性质，用抗人免疫球蛋白A、M、G抗体进行免疫印迹。在具有MS免疫复合物转移蛋白模式的硝酸纤维素膜上，分子量低于66 kDa时未发现免疫反应性。使用纯化的人髓磷脂、转移到固定化膜上的MS免疫复合物以及抗人髓磷脂碱性蛋白抗体，仅在纯化的人髓磷脂碱性蛋白上观察到免疫反应性。14 - 16 kDa的小蛋白以及38 kDa、43 kDa的其他蛋白无免疫反应性。MS免疫复合物中这些额外蛋白性质的鉴定正在进行中。
