Dasgupta M K, Catz I, Warren K G, McPherson T A, Dossetor J B, Carnegie P R
Can J Neurol Sci. 1983 Nov;10(4):239-43. doi: 10.1017/s0317167100045078.
Myelin basic protein (MBP) is an antigenic component of circulating immune complexes (CIC) in patients with multiple sclerosis (MS). Immune complexes were isolated from the sera by adsorption to Raji cells and then acid eluted. Final identification of MBP from Raji eluates was done by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by MBP radioimmunoassay (RIA) of gel eluates and by an immunoblot technique.
髓鞘碱性蛋白（MBP）是多发性硬化症（MS）患者循环免疫复合物（CIC）中的一种抗原成分。通过吸附到Raji细胞从血清中分离免疫复合物，然后进行酸洗脱。从Raji洗脱液中最终鉴定MBP是通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），随后对凝胶洗脱液进行MBP放射免疫测定（RIA）以及采用免疫印迹技术来完成的。
