Kumakura M
Department of Bioscience, Nishi-Tokyo University, Yamanashi, Japan.
Radiat Environ Biophys. 1993;32(1):41-6. doi: 10.1007/BF01213129.
Effect of irradiation dose on the production of cellulase and amylase related enzymes in Trichoderma reesei was studied, in which post-irradiation time response pattern was measured. The damage of the cells irradiated with certain irradiation doses (1.40 +/- 0.20 x 10(5), 2.20 +/- 0.10 x 10(5), 3.00 +/- 0.50 x 10(5) and 3.50 +/- 0.20 x 10(5) rad) was rapidly recovered. The increased enzyme production in the culture of the irradiated cells resulted from the recovery of radiation damage after irradiation. The function of cell growth was not affected by irradiation below dose of 5 x 10(5) rad, though the function of enzyme synthesis was drastically affected.
研究了辐照剂量对里氏木霉中纤维素酶和淀粉酶相关酶产生的影响,并测定了辐照后时间响应模式。用一定辐照剂量(1.40±0.20×10⁵、2.20±0.10×10⁵、3.00±0.50×10⁵和3.50±0.20×10⁵拉德)辐照的细胞损伤迅速恢复。辐照后细胞培养物中酶产量的增加是由于辐照后辐射损伤的恢复。低于5×10⁵拉德剂量的辐照对细胞生长功能没有影响,尽管酶合成功能受到了极大影响。