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辐射对里氏木霉中酶产生的剂量依赖性。

Dose-dependency of radiation on enzyme production in Trichoderma reesei.

作者信息

Kumakura M

机构信息

Department of Bioscience, Nishi-Tokyo University, Yamanashi, Japan.

出版信息

Radiat Environ Biophys. 1993;32(1):41-6. doi: 10.1007/BF01213129.

DOI:10.1007/BF01213129
PMID:7681603
Abstract

Effect of irradiation dose on the production of cellulase and amylase related enzymes in Trichoderma reesei was studied, in which post-irradiation time response pattern was measured. The damage of the cells irradiated with certain irradiation doses (1.40 +/- 0.20 x 10(5), 2.20 +/- 0.10 x 10(5), 3.00 +/- 0.50 x 10(5) and 3.50 +/- 0.20 x 10(5) rad) was rapidly recovered. The increased enzyme production in the culture of the irradiated cells resulted from the recovery of radiation damage after irradiation. The function of cell growth was not affected by irradiation below dose of 5 x 10(5) rad, though the function of enzyme synthesis was drastically affected.

摘要

研究了辐照剂量对里氏木霉中纤维素酶和淀粉酶相关酶产生的影响,并测定了辐照后时间响应模式。用一定辐照剂量(1.40±0.20×10⁵、2.20±0.10×10⁵、3.00±0.50×10⁵和3.50±0.20×10⁵拉德)辐照的细胞损伤迅速恢复。辐照后细胞培养物中酶产量的增加是由于辐照后辐射损伤的恢复。低于5×10⁵拉德剂量的辐照对细胞生长功能没有影响,尽管酶合成功能受到了极大影响。

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本文引用的文献

1
Cellulase production from immobilized growing cell composites prepared by radiation polymerization.
Biotechnol Bioeng. 1984 Jan;26(1):17-21. doi: 10.1002/bit.260260105.
2
Bioconversion of hemicellulose: aspects of hemicellulase production by Trichoderma reesei QM 9414 and enzymic saccharification of hemicellulose.半纤维素的生物转化:里氏木霉QM 9414产生半纤维素酶的相关方面及半纤维素的酶促糖化
Biotechnol Bioeng. 1983 Apr;25(4):1127-46. doi: 10.1002/bit.260250419.
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Radiation effects of membranes. I. Cellular permeability and cell survival.膜的辐射效应。I. 细胞通透性与细胞存活
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Some aspects of radiation effects on cell membranes.辐射对细胞膜影响的一些方面。
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The initial part of the survival curve. Implications for low-dose, low-dose-rate radiation responses.生存曲线的起始部分。对低剂量、低剂量率辐射反应的影响。
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Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 1. Separation, purification and physico-chemical characterization of five endo-1,4-beta-glucanases.粉状孢子丝菌(木栖金孢子菌)用于分解纤维素的细胞外酶系统。1. 五种内切-1,4-β-葡聚糖酶的分离、纯化及理化特性
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