Wang Bucheng, Jiang Lu, Bai Hechao, Yong Qiang, Yu Shiyuan
Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.
Biotechnol Lett. 2017 Oct;39(10):1493-1498. doi: 10.1007/s10529-017-2379-1. Epub 2017 Jun 13.
To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals.
Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3.5-4.5 g l.
Enzyme recovery at regular intervals during fed-batch cellulase cultivation could protect the enzyme in the culture broth and enhance the enzyme production when the fungal cell concentration is maintained in a reasonable range.
通过定期部分回收酶来保护分批补料纤维素酶生产过程中的酶。
每隔1、2或3天对胞外酶进行部分回收。同时去除菌丝体以避免污染。收获的纤维素酶总量增加了24% - 62%，β-葡萄糖苷酶增加了22% - 76%。在发酵罐培养中，每天用15%的培养液回收酶时，收获的纤维素酶总量和β-葡萄糖苷酶活性分别增加了43%和58%，真菌细胞浓度维持在3.5 - 4.5 g/l。
在分批补料纤维素酶培养过程中定期回收酶，可以保护培养液中的酶，并在真菌细胞浓度保持在合理范围内时提高酶产量。
