Hirano T, Manabe T, Tobe T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Dig Dis Sci. 1993 Apr;38(4):660-4. doi: 10.1007/BF01316797.
We evaluated the protective effect and the mechanism of action of the trypsin inhibitor, urinastatin, extracted from human urine, in experimental acute pancreatitis induced by a supramaximal dose of cerulein (5 micrograms/kg/hr for 3.5 hr). Urinastatin in a dose of 10,000 units/kg/hr was given by three different methods of continuous infusion: (1) 2 hr before and during cerulein infusion, (2) only during cerulein infusion, and (3) starting 1 hr after the beginning of cerulein infusion and continued for 3.5 hr. In protocol 1 and 2 urinastatin was significantly more protective than in 3. In protocol 1 urinastatin was very protective in all parameters tested (serum amylase level, pancreatic water and amylase content, distribution of lysosomal enzymes, cellular and lysosomal fragility). These results suggest that the administration of urinastatin before and during cerulein infusion may suppress the pathogenesis and evolution of pancreatitis by inhibiting the chain reaction of pancreatic enzyme activation closely related to redistribution of lysosomal enzyme and lysosomal fragility.
我们评估了从人尿中提取的胰蛋白酶抑制剂乌司他丁，在由超最大剂量雨蛙素（5微克/千克/小时，持续3.5小时）诱导的实验性急性胰腺炎中的保护作用及其作用机制。以10,000单位/千克/小时的剂量通过三种不同的持续输注方法给予乌司他丁：（1）在雨蛙素输注前2小时及输注期间；（2）仅在雨蛙素输注期间；（3）在雨蛙素输注开始后1小时开始并持续3.5小时。在方案1和2中，乌司他丁的保护作用明显强于方案3。在方案1中，乌司他丁在所有测试参数（血清淀粉酶水平﹑胰腺含水量和淀粉酶含量﹑溶酶体酶分布﹑细胞和溶酶体脆性）方面都具有很强的保护作用。这些结果表明，在雨蛙素输注前及输注期间给予乌司他丁，可能通过抑制与溶酶体酶重新分布和溶酶体脆性密切相关的胰酶激活连锁反应，来抑制胰腺炎的发病机制和发展。
