前列腺素E2对从兔肾分离出的远曲小管中激动剂刺激的环磷酸腺苷（cAMP）积累的影响。
Effect of prostaglandin E2 on agonist-stimulated cAMP accumulation in the distal convoluted tubule isolated from the rabbit kidney.
作者信息
Griffiths N M, Brick-Ghannam C, Siaume-Perez S, Chabardès D
机构信息
Laboratoire de Physiologie Cellulaire, URA 219 CNRS, Collège de France, Paris.
出版信息
Pflugers Arch. 1993 Mar;422(6):577-84. doi: 10.1007/BF00374005.
The effects of calcitonin, vasoactive intestinal peptide (VIP), parathyroid hormone (PTH) and isoprenaline on intracellular cAMP accumulation were determined in the distal tubule (DCT) microdissected from collagenase-treated rabbit kidney. In DCTb (the initial "bright" portion) calcitonin (10 ng/ml) elicited a highly reproducible response 203.7 +/- 19.1 fmol cAMP mm-1 4 min-1 (SE,N = 13) whereas VIP-induced cAMP accumulation was less and more variable from one experiment to another (1 microM, 97.2 +/- 17.8 fmol mm-1 4 min-1, SE, N = 12). When used in combination, these two agonists were non-additive, indicating stimulation of a single pool of cAMP in DCTb. In DCTg, ("granular") which consists of at least two cell types, PTH (100 nM) elicited a marked, reproducible accumulation of cAMP (154.3 +/- 27.0 fmol mm-1 4 min-1; SE, N = 5). Isoprenaline (1 microM) and VIP (1 microM) induced much smaller increases in cAMP levels 20.9 +/- 2.7 and 29.4 +/- 4.1 fmol mm-1 4 min-1 (SE, N = 5) respectively, and, when used in combination, were non-additive, demonstrating that VIP and isoprenaline are active on the same cell type. In DCTb, prostaglandin E2 (PGE2) inhibited both calcitonin- and VIP-stimulated cAMP accumulation (calcitonin 57.8 +/- 2.7% inhibition, SE, N = 16; VIP, 80.6 +/- 2.1% inhibition, SE, N = 5). The EC50 values for calcitonin were 1.21 +/- 0.33 ng/ml and 1.83 +/- 0.25 ng/ml (SD, N = 3) in the absence and presence of PGE2 (300 nM) respectively with an IC50 for PGE2 of 26.3 +/- 6.3 nM (SE, N = 4).(ABSTRACT TRUNCATED AT 250 WORDS)
在从经胶原酶处理的兔肾中显微分离出的远曲小管（DCT）中，测定了降钙素、血管活性肠肽（VIP）、甲状旁腺激素（PTH）和异丙肾上腺素对细胞内cAMP积累的影响。在DCTb（初始的“明亮”部分）中，降钙素（10 ng/ml）引发了高度可重复的反应，4分钟时为203.7±19.1 fmol cAMP·mm⁻¹·min⁻¹（标准误，N = 13），而VIP诱导的cAMP积累较少，且不同实验之间变化更大（1 μM时，为97.2±17.8 fmol·mm⁻¹·min⁻¹，标准误，N = 12）。当这两种激动剂联合使用时，它们是非相加性的，表明在DCTb中刺激的是单一的cAMP池。在由至少两种细胞类型组成的DCTg（“颗粒状”部分）中，PTH（100 nM）引发了显著的、可重复的cAMP积累（154.3±27.0 fmol·mm⁻¹·min⁻¹；标准误，N = 5）。异丙肾上腺素（1 μM）和VIP（1 μM）分别使cAMP水平升高较小，为20.9±2.7和29.4±4.1 fmol·mm⁻¹·min⁻¹（标准误，N = 5），并且联合使用时是非相加性的，表明VIP和异丙肾上腺素作用于同一细胞类型。在DCTb中，前列腺素E2（PGE2）抑制降钙素和VIP刺激的cAMP积累（降钙素抑制率为57.8±2.7%，标准误，N = 16；VIP抑制率为80.6±2.1%，标准误，N = 5）。在不存在和存在PGE2（300 nM）时，降钙素的EC50值分别为1.21±0.33 ng/ml和1.83±0.25 ng/ml（标准差，N = 3），PGE2的IC50为26.3±6.3 nM（标准误，N = 4）。（摘要截短为250字）
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