Martín de las Mulas J, Espinosa de los Monteros A, Bautista M J, Gómez-Villamandos J C, Morales C
Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Spain.
J Comp Pathol. 1994 Nov;111(4):365-81. doi: 10.1016/s0021-9975(05)80095-7.
Thirty-seven feline and 38 human spontaneous mammary gland carcinomas were studied immunohistochemically. Commercially available antibodies directed against high and low molecular weight keratins (RCK-102 and NCL-5D3), vimentin, desmin, glial fibrillary acidic protein (GFAP), neurofilament (NF) proteins and muscle actin (HHF35) were used in the avidin biotin peroxidase complex (ABC) technique on formalin-fixed paraffin wax-embedded tumour tissue samples. Healthy feline and human mammary gland tissue adjacent to the neoplasms was also examined. The distribution pattern of intermediate filament proteins and muscle actin was comparable in healthy mammary gland tissue of the two species: both RCK-102 and NCL-5D3 antibodies reacted with luminal epithelial cells of ducts and acini, but basal/myoepithelial cells were stained by RCK-102 exclusively. In addition, basal/myoepithelial cells expressed vimentin and muscle actin in both species, and GFAP was found in some feline basal/myoepithelial cells. No immunoreactivity to desmin and NF proteins was observed. Feline mammary gland carcinoma cells reacted with RCK-102 (89%), NCL-5D3 (62%), vimentin (76%) and GFAP (30%) antibodies, while human mammary gland carcinoma cells reacted with RCK-102 (95%), NCL-5D3 (100%) and vimentin (13%) antibodies. HHF35 immunoreactivity was observed in stromal cells only. These results indicate that mammary gland carcinomas of both species share a heterogeneous immunophenotype with respect to intermediate filament proteins, which adds to the list of known similarities between mammary gland carcinomas of both species.
对37例猫自发性乳腺癌和38例人类自发性乳腺癌进行了免疫组织化学研究。使用针对高分子量和低分子量角蛋白（RCK - 102和NCL - 5D3）、波形蛋白、结蛋白、胶质纤维酸性蛋白（GFAP）、神经丝（NF）蛋白和肌动蛋白（HHF35）的市售抗体，采用抗生物素蛋白 - 生物素过氧化物酶复合物（ABC）技术，对福尔马林固定、石蜡包埋的肿瘤组织样本进行检测。还检查了肿瘤附近的健康猫和人类乳腺组织。两种物种健康乳腺组织中中间丝蛋白和肌动蛋白的分布模式具有可比性：RCK - 102和NCL - 5D3抗体均与导管和腺泡的腔上皮细胞反应，但基底/肌上皮细胞仅被RCK - 102染色。此外，两种物种的基底/肌上皮细胞均表达波形蛋白和肌动蛋白，在一些猫的基底/肌上皮细胞中发现了GFAP。未观察到对结蛋白和NF蛋白的免疫反应性。猫乳腺癌细胞与RCK - 102（89%）、NCL - 5D3（62%）、波形蛋白（76%）和GFAP（30%）抗体反应，而人类乳腺癌细胞与RCK - 102（95%）、NCL - 5D3（100%）和波形蛋白（13%）抗体反应。仅在基质细胞中观察到HHF35免疫反应性。这些结果表明，两种物种的乳腺癌在中间丝蛋白方面具有异质性免疫表型，这增加了两种物种乳腺癌之间已知相似性的列表。
