Destexhe E, Lespagnard L, Degeyter M, Heymann R, Coignoul F
Pathology Department, Faculté de Médecine Vétérinaire, Université de Liège, Belgium.
Vet Pathol. 1993 Mar;30(2):146-54. doi: 10.1177/030098589303000207.
Fifty-eight formalin-fixed paraffin-embedded canine mammary tumors, 19 malignant and 39 benign, were used in this study. Tumors were obtained from dogs submitted for surgical resection of lesions at private veterinary practices in Brussels or from the surgery unit of the Faculty of Veterinary Medicine, University of Liège. Immunohistochemical evaluation was performed, using monoclonal antibodies directed against keratins 8-18 and 19, vimentin, desmin, and alpha-actin and polyclonal antibodies directed against high-molecular-weight keratins and S-100 protein. The main cell types, epithelial, myoepithelial, and connective, were identified, and myoepithelial cells represented the major component of most tumors, both benign and malignant. Myoepithelial cells had five patterns: resting and proliferative suprabasal cells, spindle and star-shaped interstitial cells, and cartilage. Reactivity to keratin 19, vimentin, alpha-actin, and S-100 protein suggested a progressive transformation from resting cells to cartilage. Epithelial cell reactivities were limited to keratins; only keratinized cells were positive for polyclonal keratins. Myofibroblasts were positive for both vimentin and alpha-actin, and connective tissue cells were positive for vimentin. Myoepithelial cells appeared to be the major component of carcinomas, justifying reevaluation and simplification of histomorphologic classifications, with a "pleomorphic carcinoma" group including all carcinomas except squamous, mucinous, and comedo carcinomas. Immunohistochemical evaluation, in addition to routine hematoxylin and eosin histopathologic evaluation is recommended for precise classification of canine mammary tumors.
本研究使用了58个经福尔马林固定、石蜡包埋的犬乳腺肿瘤，其中19个为恶性，39个为良性。肿瘤取自于在布鲁塞尔的私人兽医诊所接受病变手术切除的犬只，或列日大学兽医学院外科。采用针对角蛋白8 - 18和19、波形蛋白、结蛋白和α - 肌动蛋白的单克隆抗体以及针对高分子量角蛋白和S - 100蛋白的多克隆抗体进行免疫组织化学评估。确定了主要细胞类型，即上皮细胞、肌上皮细胞和结缔组织细胞，肌上皮细胞是大多数肿瘤（包括良性和恶性）的主要成分。肌上皮细胞有五种模式：静止和增殖的基底上层细胞、梭形和星形间质细胞以及软骨。对角蛋白19、波形蛋白、α - 肌动蛋白和S - 100蛋白的反应表明从静止细胞到软骨的渐进性转变。上皮细胞反应仅限于角蛋白；只有角化细胞对多克隆角蛋白呈阳性。肌成纤维细胞对波形蛋白和α - 肌动蛋白均呈阳性，结缔组织细胞对波形蛋白呈阳性。肌上皮细胞似乎是癌的主要成分，这证明了对组织形态学分类进行重新评估和简化的合理性，“多形性癌”组包括除鳞状、黏液性和粉刺癌以外的所有癌。除了常规苏木精和伊红组织病理学评估外，建议进行免疫组织化学评估以精确分类犬乳腺肿瘤。
