Girjes A A, Ellis W A, Carrick F N, Lavin M F
Department of Zoology, University of Queensland, St. Lucia, Brisbane, Australia.
FEMS Immunol Med Microbiol. 1993 Jan;6(1):21-30. doi: 10.1111/j.1574-695X.1993.tb00299.x.
Western-blot analysis was used to study the reaction of koala antisera, two specific polyclonal antibodies and one monoclonal antibody, with chlamydial antigens in koalas infected with Chlamydia psittaci. The koala sera recognized four C. psittaci surface antigens, corresponding to the major outer membrane protein (39.5 kDa), 31 kDa protein, 18 kDa protein and lipopolysaccharide. The S25-23 LPS specific monoclonal antibody inhibited chlamydial infection (55-67%) with both koala strains (type I and type II). Both koala antiserum and rabbit polyclonal antibodies against either type of chlamydia significantly reduced the number of infected cells resulting from type II infections at a dilution of 1 in 20. Rabbit antiserum against type II was effective in neutralizing infection by type II elementary bodies, but was less effective against type I infection. In addition, no koala antiserum was effective in neutralizing type I infection.
采用蛋白质免疫印迹分析来研究考拉抗血清、两种特异性多克隆抗体和一种单克隆抗体与感染鹦鹉热衣原体的考拉体内衣原体抗原的反应。考拉血清识别出四种鹦鹉热衣原体表面抗原，分别对应主要外膜蛋白（39.5 kDa）、31 kDa蛋白、18 kDa蛋白和脂多糖。S25 - 23 LPS特异性单克隆抗体对两种考拉菌株（I型和II型）的衣原体感染均有抑制作用（55 - 67%）。考拉抗血清和针对任一型衣原体的兔多克隆抗体在稀释至1:20时，均能显著减少II型感染所导致的感染细胞数量。抗II型的兔抗血清能有效中和II型原体的感染，但对I型感染的效果较差。此外，没有考拉抗血清能有效中和I型感染。
