Drexler H G
German Collection of Microorganisms & Cell Cultures, Braunschweig.
Leuk Lymphoma. 1993 Jan;9(1-2):1-25. doi: 10.3109/10428199309148499.
The relative scarcity of Hodgkin (H) and Reed-Sternberg (RS) cells within biopsies from cases with Hodgkin's disease (HD) is an impediment to the analysis of the nature and function of these cells. Continuous cell lines as uniform and permanently available sources of cells provide a valid alternative. Development of HD cell lines has proven to be rather difficult when compared with the results on leukemia and Non-Hodgkin lymphoma cells. Only a few cell lines containing cells that resemble in-vivo H-RS cells have been established. Because the in-vitro culture conditions favor the self-propagation of residual normal cells, e.g. Epstein-Barr virus transformed B-lymphoblastoid cells or monocyte/macrophage monolayers, early attempts at culturing HD tissue resulted mainly in the generation of such cell lines. Even for the bona fide HD cell lines it is difficult to prove that the immortalized cells originated from an H-RS cell. These 13 HD cell lines have been extensively characterized in a large variety of aspects. These data have resulted in widely varying conclusions about the nature of the cell lines. It is apparent that all HD cell lines are unique among hematopoietic cell lines and are also different from one another. No conclusive evidence towards the origin of the cells has been obtained for some cell lines, while others could be operationally, albeit not always unequivocally, assigned to the T- or B-cell or monocyte-macrophage lineages. The overall phenotypes are often not concordant with those of normal hematopoietic cells; some cell lines show clearly mixed lineage attributes. The artifactual expansion of non-HRS cells in culture and the acquisition or loss of certain properties during the adaptation to culture systems cannot be excluded. There was also a bias for the establishment of cell lines from cases with advanced clinical stages, nodular sclerosing subtype and pleural effusions. The extensive analysis of a few cell lines has provided a wealth of information useful for the understanding of the biology of H-RS cells. The striking heterogeneity could be reflective of a biologically heterogeneous disease.
在霍奇金淋巴瘤（HD）病例的活检组织中，霍奇金（H）细胞和里德 - 斯腾伯格（RS）细胞相对稀少，这阻碍了对这些细胞的性质和功能进行分析。连续细胞系作为均匀且可永久获取的细胞来源提供了一个有效的替代方案。与白血病和非霍奇金淋巴瘤细胞系的研究成果相比，HD细胞系的建立已被证明相当困难。仅建立了少数几个含有类似于体内H - RS细胞的细胞系。由于体外培养条件有利于残留正常细胞的自我增殖，例如爱泼斯坦 - 巴尔病毒转化的B淋巴母细胞系或单核细胞/巨噬细胞单层，早期培养HD组织的尝试主要产生了这类细胞系。即使对于真正的HD细胞系，也很难证明永生化细胞起源于H - RS细胞。这13个HD细胞系已在诸多方面进行了广泛的特征描述。这些数据得出了关于细胞系性质的广泛不同的结论。显然，所有HD细胞系在造血细胞系中都是独特的，并且彼此之间也存在差异。对于一些细胞系，尚未获得关于细胞起源的确凿证据，而其他细胞系尽管并非总是明确无误，但在操作上可归为T细胞或B细胞或单核细胞 - 巨噬细胞谱系。总体表型往往与正常造血细胞的表型不一致；一些细胞系表现出明显的混合谱系特征。不能排除培养中非HRS细胞的人为扩增以及在适应培养系统过程中某些特性的获得或丧失。从临床晚期、结节硬化亚型和胸腔积液病例中建立细胞系也存在偏差。对少数细胞系的广泛分析提供了大量有助于理解H - RS细胞生物学的信息。显著的异质性可能反映了一种生物学上异质性的疾病。
