Tørsløv J
Water Quality Institute, Agern Alle 11, Hørsholm, Denmark.
Ecotoxicol Environ Saf. 1993 Feb;25(1):33-40. doi: 10.1006/eesa.1993.1004.
Bacterial test systems that are used to assess the environmental risk of chemicals show considerable differences in sensitivity to toxic chemicals. This variation may be caused by differences in sensitivity between species and test parameters of the test systems. In this study, the differences in sensitivity of three test parameters, growth, esterase activity, and dehydrogenase activity, using Pseudomonas fluorescens as the test organism, were studied. The effect of eight substances was tested: pentachlorophenol, 2,4-dichlorophenol, aniline, trichloroethylene, nonylphenolethoxylate, tetrapropylene benzene sulphonate, copper, and nickel. None of the three test parameters was the most sensitive to all substances, although dehydrogenase activity showed a high sensitivity in most cases. The observed differences in sensitivity among the three test parameters were of the same order of magnitude as those previously observed for different strains of bacteria. It was concluded that the choice of test parameter is just as important as the choice of test species to the sensitivity of a test system. The differences in sensitivity between test parameters and between species may account for a great deal of the observed variation between test results obtained with different methods.
用于评估化学品环境风险的细菌测试系统对有毒化学品的敏感性存在显著差异。这种差异可能是由测试系统的物种敏感性和测试参数的差异所导致的。在本研究中，以荧光假单胞菌作为测试生物，研究了生长、酯酶活性和脱氢酶活性这三个测试参数的敏感性差异。测试了八种物质的影响：五氯苯酚、2,4-二氯苯酚、苯胺、三氯乙烯、壬基酚聚氧乙烯醚、四聚丙烯苯磺酸盐、铜和镍。尽管脱氢酶活性在大多数情况下表现出高敏感性，但这三个测试参数中没有一个对所有物质都是最敏感的。观察到的这三个测试参数之间的敏感性差异与先前在不同细菌菌株中观察到的差异处于同一数量级。得出的结论是，测试参数的选择与测试物种的选择对测试系统的敏感性同样重要。测试参数之间以及物种之间的敏感性差异可能是不同方法获得的测试结果之间观察到的大量差异的原因。
