Tørsløv J, Lindgaard-Jørgensen P
Water Quality Institute, Hørsholm, Denmark.
Ecotoxicol Environ Saf. 1994 Oct;29(1):86-92. doi: 10.1016/0147-6513(94)90033-7.
Industrial wastewater often contains a mixture of chemical substances and knowledge of joint action of toxicants is therefore important when the toxicity of an effluent is evaluated or reduction of the toxicity is needed. In this study, the joint action of three pairs of toxicants, selected on the basis of their expected different modes of toxic actions, was tested for inhibition of the growth of the bacteria Pseudomonas fluorescens: pentachlorophenol and aniline, the surfactants nonylphenolethoxylate and tetrapropylenebenzenesulfonate, and pentachlorophenol and 2, 4-dichlorophenol. The joint effect of pentachlorophenol and aniline did not differ significantly from additivity, whereas less than additive responses were observed for mixtures of nonylphenolethoxylate and tetrapropylenbenzenesulfonate. A more than additive response was observed for mixtures of pentachlorophenol and 2,4-dichlorophenol at some concentration levels, while at others additive responses were found. It was concluded that joint actions other than additivity may occur between commonly known toxic substances, and that the modes of toxic action of the substances studied can explain the different types of joint action observed in this study. Further, test strategy followed proved useful in the evaluation of the joint toxicity of binary mixtures.
工业废水通常含有多种化学物质，因此在评估废水毒性或需要降低毒性时，了解毒物的联合作用非常重要。在本研究中，基于预期的不同毒性作用模式选择了三对毒物，测试它们对荧光假单胞菌生长的抑制作用：五氯苯酚和苯胺、表面活性剂壬基酚聚氧乙烯醚和四聚丙烯苯磺酸盐，以及五氯苯酚和2,4-二氯苯酚。五氯苯酚和苯胺的联合效应与相加作用无显著差异，而壬基酚聚氧乙烯醚和四聚丙烯苯磺酸盐混合物的反应小于相加作用。在某些浓度水平下，五氯苯酚和2,4-二氯苯酚混合物的反应大于相加作用，而在其他浓度水平下则为相加作用。得出的结论是，常见毒物之间可能会出现相加作用以外的联合作用，并且所研究物质的毒性作用模式可以解释本研究中观察到的不同类型的联合作用。此外，所采用的测试策略被证明在评估二元混合物的联合毒性方面是有用的。
