鉴定由b型流感嗜血杆菌P1特异性单克隆抗体识别的表面暴露B细胞表位。
Identification of surface-exposed B-cell epitopes recognized by Haemophilus influenzae type b P1-specific monoclonal antibodies.
作者信息
Panezutti H, James O, Hansen E J, Choi Y, Harkness R E, Klein M H, Chong P
机构信息
Connaught Centre for Biotechnology Research, Willowdale, Ontario, Canada.
出版信息
Infect Immun. 1993 May;61(5):1867-72. doi: 10.1128/iai.61.5.1867-1872.1993.
Abstract
A panel of P1 synthetic peptides was synthesized to map the surface-exposed epitopes of Haemophilus influenzae type b outer membrane protein P1 recognized by three murine monoclonal antibodies (MAbs 7C8, 3E12, and 6B1). By using peptide-specific enzyme-linked immunosorbent assays, MAbs 6B1, 7C8, and 3E12 were shown to recognize distinct epitopes localized within residues 60 to 88, 165 to 193, and 400 to 437 of mature P1, respectively. Since MAb 7C8 was shown previously to be protective against certain H. influenzae type b subtypes in the infant rat model of bacteremia, its cognate epitope was further characterized by using truncated peptide analogs. Fine mapping of the 7C8 epitope by competitive inhibition studies revealed that it was localized within residues 184 and 193.
摘要
合成了一组P1合成肽，以绘制被三种鼠单克隆抗体（单克隆抗体7C8、3E12和6B1）识别的b型流感嗜血杆菌外膜蛋白P1的表面暴露表位。通过使用肽特异性酶联免疫吸附测定，显示单克隆抗体6B1、7C8和3E12分别识别位于成熟P1的60至88、165至193和400至437位残基内的不同表位。由于先前已证明单克隆抗体7C8在幼鼠菌血症模型中对某些b型流感嗜血杆菌亚型具有保护作用，因此通过使用截短的肽类似物对其同源表位进行了进一步表征。通过竞争性抑制研究对7C8表位进行精细定位，结果显示它位于184和193位残基内。
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本文引用的文献
1
Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications.流感嗜血杆菌疾病分离株的外膜蛋白组成：致病和流行病学意义
Infect Immun. 1980 Dec;30(3):709-17. doi: 10.1128/iai.30.3.709-717.1980.
2
Prediction of protein antigenic determinants from amino acid sequences.从氨基酸序列预测蛋白质抗原决定簇。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8. doi: 10.1073/pnas.78.6.3824.
3
The pathogenicity of Haemophilus influenzae.流感嗜血杆菌的致病性。
J Med Microbiol. 1984 Aug;18(1):1-16. doi: 10.1099/00222615-18-1-1.
4
Immunoprotection of rats against Haemophilus influenzae type B disease mediated by monoclonal antibody against a haemophilus outer-membrane protein.抗B型流感嗜血杆菌外膜蛋白单克隆抗体介导的大鼠抗B型流感嗜血杆菌疾病的免疫保护作用
Lancet. 1982 Feb 13;1(8268):366-8. doi: 10.1016/s0140-6736(82)91394-0.
5
The immune response to Epstein-Barr nuclear antigen: conformational and structural features of antibody binding to synthetic peptides.针对爱泼斯坦-巴尔核抗原的免疫反应：抗体与合成肽结合的构象和结构特征。
Mol Immunol. 1984 Nov;21(11):1047-54. doi: 10.1016/0161-5890(84)90114-7.
6
Protection of infant rats from Haemophilus influenzae type b infection by antiserum to purified outer membrane protein a.用抗纯化外膜蛋白a血清保护幼鼠免受b型流感嗜血杆菌感染。
Infect Immun. 1987 Nov;55(11):2612-8. doi: 10.1128/iai.55.11.2612-2618.1987.
7
Prospects for prevention of Haemophilus influenzae type b disease by immunization.通过免疫接种预防b型流感嗜血杆菌疾病的前景。
J Infect Dis. 1986 Mar;153(3):448-61. doi: 10.1093/infdis/153.3.448.
8
The influence of pH and ionic strength on the coating of peptides of herpes simplex virus type 1 in an enzyme-linked immunosorbent assay.
J Immunol Methods. 1988 Feb 10;106(2):239-44. doi: 10.1016/0022-1759(88)90203-7.
9
Cloning and expression in Escherichia coli of the gene encoding the heat-modifiable major outer membrane protein of Haemophilus influenzae type b.b型流感嗜血杆菌热可修饰主要外膜蛋白编码基因在大肠杆菌中的克隆与表达。
Infect Immun. 1987 Dec;55(12):2993-3000. doi: 10.1128/iai.55.12.2993-3000.1987.
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