Kristensen P, Eilertsen E, Einarsdóttir E, Ovrebø S, Haugen A
National Institute of Occupational Health, Oslo, Norway.
Mutat Res. 1993 May;302(1):33-8. doi: 10.1016/0165-7992(93)90087-c.
Experimental evidence suggests that inorganic lead may modify mutagenic events. We examined the modifying effect of lead on mutagenic events in late spermatogenesis in the dominant lethal assay. Twelve NMRI male mice were given lead chloride in the drinking water and 12 male mice received tap water without lead chloride. Cyclophosphamide (120 mg/kg b.w., i.p.) was given to six males in the lead treatment group and six males in the tap water group 1 week before mating. This resulted in four treatment groups: control, lead, cyclophosphamide, and lead plus cyclophosphamide. Cyclophosphamide given to the males (with or without lead treatment) reduced the numbers of live implants in mated females. The most prominent effect of cyclophosphamide was an increase of resorbed implants. Females mated to lead exposed male mice showed a nonsignificantly lower frequency of resorptions compared to controls. The results give no support to the hypothesis that inorganic lead may influence the mutagenicity of cyclophosphamide in the dominant lethal test.
实验证据表明无机铅可能会改变诱变事件。我们在显性致死试验中研究了铅对精子发生后期诱变事件的修饰作用。12只NMRI雄性小鼠饮用含氯化铅的水，12只雄性小鼠饮用不含氯化铅的自来水。在交配前1周，给铅处理组的6只雄性小鼠和自来水组的6只雄性小鼠腹腔注射环磷酰胺（120mg/kg体重）。这产生了四个处理组：对照组、铅处理组、环磷酰胺处理组和铅加环磷酰胺处理组。给雄性小鼠注射环磷酰胺（无论是否经过铅处理）都会减少交配雌鼠体内的活胚植入数量。环磷酰胺最显著的作用是吸收胚植入数量增加。与对照组相比，与接触铅的雄性小鼠交配的雌鼠吸收胚的频率略低，但差异不显著。这些结果不支持无机铅可能在显性致死试验中影响环磷酰胺诱变性的假说。
