Chart H, Willshaw G A, Cheasty T, Rowe B
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, UK.
J Appl Bacteriol. 1993 May;74(5):583-7.
Citrobacter freundii strain E69366 was detected in a colony immunoassay with a rabbit antiserum prepared to a strain of Escherichia coli belonging to serogroup O157. Lipopolysaccharide (LPS) was shown to contain the epitope(s) involved in antibody binding. LPS prepared from strain E69366, with hot phenol contained core-LPS that migrated to the water-phase and long-chain LPS that separated into the phenol-phase. A total of 36 strains of Cit. freundii were analysed for their LPS profiles by SDS-PAGE. Sixteen could be allocated into five groups (A(3), B(7), C(2), D(2) and E(2)) based on similarities in LPS profile. The remaining strains either expressed unique SDS-PAGE profiles (18) or did not express long-chain LPS (2). All strains with a profile 'A' reacted with the antiserum prepared to E. coli O157, whereas those with other profiles did not. The two strains of Cit. freundii expressing LPS profiles designated 'E' reacted with an antiserum prepared in rabbits to E. coli O45.
在一项菌落免疫测定中，用针对属于O157血清群的大肠杆菌菌株制备的兔抗血清检测到弗氏柠檬酸杆菌E69366菌株。结果表明，脂多糖（LPS）含有参与抗体结合的表位。用热酚从E69366菌株制备的LPS含有迁移到水相的核心LPS和分离到酚相的长链LPS。通过SDS-PAGE分析了总共36株弗氏柠檬酸杆菌的LPS谱。基于LPS谱的相似性，16株可分为五组（A(3)、B(7)、C(2)、D(2)和E(2)）。其余菌株要么表现出独特的SDS-PAGE谱（18株），要么不表达长链LPS（2株）。所有具有“A”谱的菌株都与针对大肠杆菌O157制备的抗血清发生反应，而具有其他谱的菌株则不反应。两株表达指定为“E”的LPS谱的弗氏柠檬酸杆菌与用兔制备的针对大肠杆菌O45的抗血清发生反应。
