Mansfield L P, Billett E, Olsen E, Forsythe S J
Department of Life Sciences, Nottingham Trent University, UK.
Lett Appl Microbiol. 1996 Aug;23(2):104-6. doi: 10.1111/j.1472-765x.1996.tb00041.x.
The lipopolysaccharide antigenicity of 22 Salmonella strains (representing nine serogroups) and four non-salmonellae Enterobacteriaceae to the Salmonella genus specific monoclonal antibody M105 was analysed. The monoclonal antibody M105 reacted with all 22 Salmonella strains. Probing SDS-PAGE separated LPS molecules with MAb M105 revealed that the antibody reacted with the core region of all Salmonella serovars. However, no reaction was obtained to the long-chain LPS of serovars O (Salm. adelaide and Salm. ealing), C1 (Salm. infantis, Salm. livingstone and Salm. virchow) or Salm. arizonae. It is plausible that the presence of a second core antigenic type results in the lack of reaction between long-chain LPS and the Salmonella genus specific monoclonal antibody M105.
分析了22株沙门氏菌（代表9个血清群）和4株非沙门氏菌肠杆菌科细菌对沙门氏菌属特异性单克隆抗体M105的脂多糖抗原性。单克隆抗体M105与所有22株沙门氏菌均发生反应。用单克隆抗体M105探测SDS-PAGE分离的脂多糖分子显示，该抗体与所有沙门氏菌血清型的核心区域发生反应。然而，血清型O（阿德莱德沙门氏菌和伊灵沙门氏菌）、C1（婴儿沙门氏菌、利文斯通沙门氏菌和维尔乔沙门氏菌）或亚利桑那沙门氏菌的长链脂多糖未出现反应。长链脂多糖与沙门氏菌属特异性单克隆抗体M105之间缺乏反应可能是由于存在第二种核心抗原类型。
