Barale R, Scapoli C, Meli C, Casini D, Minunni M, Marrazzini A, Loprieno N, Barrai I
Dipartimento di Biologia Evolutiva, Università di Ferrara, Italy.
Mutat Res. 1993 Jun;300(1):15-28. doi: 10.1016/0165-1218(93)90135-z.
The cytogenetic effects of three benzimidazoles, i.e., benomyl, methyl thiophanate and methyl 2-benzimidazolecarbamate (MBC), were studied in mouse bone marrow cells by analyzing three genetic endpoints: micronuclei, structural chromosome aberrations plus or minus gaps, and aneugenic effects (hyperdiploidy or polyploidy). In general, the effects were small, but it was observed that benomyl and MBC significantly induced micronuclei as well as aneugenic effects, hyperdiploidy (no metaphases with more than one or two extra chromosomes, 2n + 1 or 2n + 2, were observed) and polyploidy (4n). The induction of chromosome gaps and breaks was less evident. Methyl thiophanate significantly induced micronuclei, but it was less effective than benomyl and MBC. Our results showed that micronuclei are a good indicator of aneugenic effects in mouse bone marrow cells. A curvilinear trend test has been devised to fit the curves originating from the time-dependent responses.
通过分析三个遗传终点，即微核、有或无间隙的结构染色体畸变以及非整倍体效应（超二倍体或多倍体），研究了三种苯并咪唑类药物，即苯菌灵、甲基托布津和多菌灵对小鼠骨髓细胞的细胞遗传学效应。总体而言，效应较小，但观察到苯菌灵和多菌灵显著诱导微核以及非整倍体效应、超二倍体（未观察到具有多于一条或两条额外染色体，即2n + 1或2n + 2的中期相）和多倍体（4n）。染色体间隙和断裂的诱导不太明显。甲基托布津显著诱导微核，但效果不如苯菌灵和多菌灵。我们的结果表明，微核是小鼠骨髓细胞中非整倍体效应的良好指标。已设计出一种曲线趋势检验来拟合源自时间依赖性反应的曲线。
