三正丁基锡和三苯基锡对培养的中国仓鼠细胞中化学诱导染色体畸变频率的增强作用。

Increasing effect of tri-n-butyltins and triphenyltins on the frequency of chemically induced chromosome aberrations in cultured Chinese hamster cells.

作者信息

Sasaki Y F, Yamada H, Sugiyama C, Kinae N

机构信息

Biological Laboratory, School of Science, Kwansei Gakuin University, Hyogo, Japan.

出版信息

Mutat Res. 1993 Jun;300(1):5-14. doi: 10.1016/0165-1218(93)90134-y.

DOI:10.1016/0165-1218(93)90134-y

PMID:7683769

Abstract

Organotins have been widely used as anti-fouling coatings for fishing nets and ship bottoms, and marine pollution by them has become a serious environmental problem. In this communication, the potentiating effects of three kinds of tri-n-butyltins and three thiphenyltins on chromosome aberrations were studied in Chinese hamster CHO K1 cells. None of the organotins studied showed any clastogenic activity under the experimental conditions without rat liver S9. Post-treatment with organotins, however, increased the number of breakage-type (but not exchange-type) chromatid aberrations induced by five kinds of S-phase-dependent clastogens: MMC, cisPt, 4NQO, MMS, and AMD). Enhancement of the induction of chromosome aberrations by MMC was observed when cells were treated with organotins during the G2 phase. These results suggest that organotin G2 effect causes potentiating effects. Organotins also enhanced the induction of breakage-type chromatid aberrations by clastogenic pollutants in chlorinated tap water, indicating their potential for a more realistic health risk.

摘要

有机锡已被广泛用作渔网和船底的防污涂料，它们造成的海洋污染已成为一个严重的环境问题。在本报告中，研究了三种三丁基锡和三种三苯基锡对中国仓鼠CHO K1细胞染色体畸变的增强作用。在所研究的有机锡中，在没有大鼠肝脏S9的实验条件下，没有一种显示出任何致断裂活性。然而，用有机锡进行后处理增加了由五种S期依赖性断裂剂（丝裂霉素C、顺铂、4-硝基喹啉-N-氧化物、甲基磺酸甲酯和氨基蝶呤）诱导的断裂型（而非交换型）染色单体畸变的数量。当细胞在G2期用有机锡处理时，观察到丝裂霉素C诱导的染色体畸变增强。这些结果表明，有机锡的G2效应会产生增强作用。有机锡还增强了氯化自来水中致断裂污染物诱导的断裂型染色单体畸变，表明它们存在更现实的健康风险。