Kargacin B, Klein C B, Costa M
New York University Medical Center, Nelson Institute of Environmental Medicine, NY 10016.
Mutat Res. 1993 Jun;300(1):63-72. doi: 10.1016/0165-1218(93)90141-y.
Mutagenesis of several insoluble nickel compounds--crystalline nickel sulfide NiS, nickel subsulfide Ni3S2, nickel oxides (black and green) and soluble NiCl2 was studied in three Chinese hamster cell lines--at the hprt gene of the well-defined V79 cell line, and at gpt in two transgenic derivative cell lines G12 and G10. The transgenic cell line G12 responded very strongly to the insoluble Ni compounds, such that the gpt mutagenesis was at least 20 times higher than the spontaneous mutagenesis and in some experiments was even higher. In contrast the response of the G10 cells was much lower--the mutant frequencies only increased 2-3 times over the controls. In V79 cells, NiS and NiO (black) did not induce a mutagenic response at hprt. Soluble NiCl2 also exhibited no mutagenic activity in V79 cells and induced considerably lower activity than the insoluble compounds in the transgenic G12 cells. Following vitamin E pretreatment of G12 cells for 24 h prior to nickel exposure, increased cell survival was observed for several insoluble Ni compounds whereas vitamin E had no effect on NiCl2 cytotoxicity. With vitamin E pretreatment, significantly lower mutagenic responses in G12 cells were also noted for some insoluble Ni compounds, while no such effect was observed for NiCl2.
研究了几种不溶性镍化合物（结晶硫化镍NiS、次硫化镍Ni3S2、氧化镍（黑色和绿色））和可溶性NiCl2在三种中国仓鼠细胞系中的诱变作用——在明确的V79细胞系的hprt基因处，以及在两种转基因衍生细胞系G12和G10的gpt基因处。转基因细胞系G12对不溶性镍化合物反应非常强烈，使得gpt诱变作用比自发诱变作用至少高20倍，在某些实验中甚至更高。相比之下，G10细胞的反应要低得多——突变频率仅比对照增加2至3倍。在V79细胞中，NiS和NiO（黑色）在hprt处未诱导诱变反应。可溶性NiCl2在V79细胞中也未表现出诱变活性，并且在转基因G12细胞中诱导的活性比不溶性化合物低得多。在镍暴露前对G12细胞进行24小时维生素E预处理后，观察到几种不溶性镍化合物的细胞存活率增加，而维生素E对NiCl2的细胞毒性没有影响。经过维生素E预处理后，还注意到一些不溶性镍化合物在G12细胞中的诱变反应明显降低，而NiCl2未观察到这种效果。
