Pivovarov A S, Drozdova E I, Zabolotskii D A, Myagkova G I
Department of Higher Nervous Activity, M.V. Lomonosov Moscow State University.
Neurosci Behav Physiol. 1993 Mar-Apr;23(2):176-81. doi: 10.1007/BF01189115.
The influence of three polyacetylenic analogs of natural polyenoic acids which are inhibitors of their lipoxygenase oxidation on the dynamics of the extinction of the inward current induced by repeated iontophoretic applications of acetylcholine to the soma was investigated in identified RPa3 and LPa3 of the edible snail using the method of bielectrode recording of the potential on the membrane. It was found that eicosa-5,8,11,14-tetraynoic acid (30-60 mumole/liter) and eicosa-5,8,11,14,17-pentaynoic acid (4-50 mumole/liter) decrease the amplitude of the inward current induced by application of acetylcholine, and attenuate its extinction during repeated applications. The third analog, eicosa-8,11,14-triaynoic acid, does not exert a modulating influence on the magnitude of the current and its extinction. It was hypothesized that the lipoxygenase metabolites of the polyenoic acids regulate the plasticity of the cholinoreceptors of the neurons of the edible snail. Taking the nonidentical inhibition by the compounds used of the various lipoxygenases into account, the participation in the plasticity of the cholinoreceptors of those eicosanoids which are formed from arachidonic acid under the influence of 5-lipoxygenases is most probable. At the same time, a regulatory role of the eicosanoids formed under the influence of other lipoxygenases is not excluded.
在可食用蜗牛的已鉴定RPa3和LPa3中，使用双电极记录膜电位的方法，研究了三种天然多烯酸的聚乙炔类似物（它们是脂氧合酶氧化的抑制剂）对通过向躯体重复离子电渗应用乙酰胆碱所诱导的内向电流消退动力学的影响。结果发现，二十碳-5,8,11,14-四炔酸（30 - 60微摩尔/升）和二十碳-5,8,11,14,17-五炔酸（4 - 50微摩尔/升）可降低乙酰胆碱应用所诱导的内向电流幅度，并在重复应用期间减弱其消退。第三种类似物，二十碳-8,11,14-三炔酸，对电流大小及其消退没有调节作用。据推测，多烯酸的脂氧合酶代谢产物调节可食用蜗牛神经元胆碱能受体的可塑性。考虑到所用化合物对各种脂氧合酶的抑制作用不同，最有可能是由花生四烯酸在5-脂氧合酶影响下形成的那些类二十烷酸参与了胆碱能受体的可塑性。同时，不排除其他脂氧合酶影响下形成的类二十烷酸的调节作用。
