Brownlee D J, Fairweather I, Johnston C F
School of Biology and Biochemistry, Queen's University of Belfast, Medical Biology Centre, Northern Ireland.
Parasitol Res. 1993;79(4):302-8. doi: 10.1007/BF00932186.
The localisation and distribution of neuropeptides in the peripheral nervous system of the pig roundworm Ascaris suum have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Of the 31 antisera tested, immunostaining was obtained only with antisera to peptide YY (PYY), pancreatic polypeptide (PP) and FMRFamide. Immunostaining for PYY and FMRFamide was evident in the amphidial and papillary ganglia associated with the anterior nerve ring and in the nerves from these ganglia that terminated in sensory receptors within the buccal lips of the parasite. The only peptide immunoreactivity (IR) observed in the reproductive system of either sex was that evident in the nerve supply to the distal region of the vagina in the female worm. It took the form of a well-developed plexus of parallel nerve fibres, cross-connectives and looped commissures. The nerve net diminished in the more proximal region of the vagina. PP-IR was less intense than that for PYY and FMRFamide and was more restricted in distribution, being confined to a small number of nerve fibres in the nerve supply to the vagina; it did not occur in the nerves supplying the anterior sensory receptors. The possible roles of neuropeptides in the sensory and reproductive biology of nematodes are discussed.
利用间接免疫荧光技术结合共聚焦显微镜，已确定了猪蛔虫外周神经系统中神经肽的定位和分布。在所测试的31种抗血清中，仅对肽YY（PYY）、胰多肽（PP）和FMRF酰胺的抗血清获得了免疫染色。与前神经环相关的头感器和乳突神经节以及来自这些神经节并终止于寄生虫口唇部感觉感受器的神经中，PYY和FMRF酰胺的免疫染色明显。在两性生殖系统中观察到的唯一肽免疫反应性（IR）是在雌虫阴道远端区域的神经供应中明显的免疫反应性。它表现为平行神经纤维、交叉连接和环状连合组成的发达神经丛。在阴道更近端区域，神经网减少。PP-IR比PYY和FMRF酰胺的免疫反应性弱，分布更局限，仅局限于阴道神经供应中的少数神经纤维；在前部感觉感受器的神经中未出现。讨论了神经肽在线虫感觉和生殖生物学中的可能作用。
