Storb R, Raff R F, Graham T, Appelbaum F R, Deeg H J, Schuening F G, Shulman H, Pepe M
Division of Clinical Research, University of Washington School of Medicine, Seattle.
Int J Radiat Oncol Biol Phys. 1993 May 20;26(2):275-83. doi: 10.1016/0360-3016(93)90207-c.
We explored in dogs the marrow toxicity of single dose total body irradiation delivered from two opposing 60Co sources at a rate of 10 cGy/min and compared results to those seen with total body irradiation administered in 100 cGy fractions with minimum interfraction intervals of 6 hr. Dogs were not given marrow transplants.
We found that 200 cGy single dose total body irradiation was sublethal, with 12 of 13 dogs showing hematopoietic recovery and survival. Seven of 21 dogs given 300 cGy single dose total body irradiation survived compared to 6 of 10 dogs given 300 cGy fractionated total body irradiation (p = .18). One of 28 dogs given 400 cGy single dose total body irradiation survived compared to none of six given fractionated radiation (p > .20). With granulocyte colony stimulating factor administered from day 0-21 after 400 cGy total body irradiation, most dogs survived with hematological recovery. Because of the almost uniform success with granulocyte colony stimulating factor after 400 cGy single dose total body irradiation, a study of granulocyte colony stimulating factor after 400 cGy fractionated total body irradiation was deemed not to be informative and, thus, not carried out. Additional comparisons between single dose and fractionated total body irradiation were carried out with granulocyte colony stimulating factor administered after 500 and 600 cGy of total body irradiation. As with lower doses of total body irradiation, no significant survival differences were seen between the two modes of total body irradiation, and only 3 of 26 dogs studied survived with complete hematological recovery. Overall, therefore, survival among dogs given single dose total body irradiation was not different from that of dogs given fractionated total body irradiation (p = .67). Similarly, the slopes of the postirradiation declines of granulocyte and platelet counts and the rates of their recovery in surviving dogs given equal total doses of single versus fractionated total body irradiation were indistinguishable.
Within the limitations of the experimental design, we conclude that single-dose and fractionated total body irradiation have comparable marrow toxicity in dogs.
我们在犬类中探究了以10厘戈/分钟的速率从两个相对的60钴源进行单次全身照射的骨髓毒性，并将结果与以100厘戈分次进行全身照射且分次间隔最短为6小时的情况进行比较。犬类未接受骨髓移植。
我们发现200厘戈单次全身照射是亚致死性的，13只犬中有12只显示造血恢复并存活。接受300厘戈单次全身照射的21只犬中有7只存活，而接受300厘戈分次全身照射的10只犬中有6只存活（p = 0.18）。接受400厘戈单次全身照射的28只犬中有1只存活，而接受分次照射的6只犬均未存活（p > 0.20）。在400厘戈全身照射后第0至21天给予粒细胞集落刺激因子，大多数犬存活并伴有血液学恢复。由于400厘戈单次全身照射后使用粒细胞集落刺激因子几乎均取得成功，因此认为对400厘戈分次全身照射后使用粒细胞集落刺激因子进行研究并无意义，故未开展此项研究。在500和600厘戈全身照射后给予粒细胞集落刺激因子，对单次全身照射和分次全身照射进行了额外比较。与较低剂量的全身照射一样，两种全身照射方式之间未观察到显著的生存差异，所研究的26只犬中只有3只存活并伴有完全的血液学恢复。因此，总体而言，接受单次全身照射的犬类的生存率与接受分次全身照射的犬类并无差异（p = 0.67）。同样，在接受相同总剂量的单次与分次全身照射的存活犬类中，粒细胞和血小板计数照射后下降的斜率及其恢复速率并无区别。
在实验设计的局限性范围内，我们得出结论，单次全身照射和分次全身照射在犬类中具有相当的骨髓毒性。
