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Nicotinic receptor-mediated release of noradrenaline in the human neuroblastoma SH-SY5Y.

作者信息

Vaughan P F, Kaye D F, Reeve H L, Ball S G, Peers C

机构信息

Department of Cardiovascular Studies, University of Leeds, England.

出版信息

J Neurochem. 1993 Jun;60(6):2159-66. doi: 10.1111/j.1471-4159.1993.tb03501.x.

DOI:10.1111/j.1471-4159.1993.tb03501.x
PMID:7684069
Abstract

Dimethylphenylpiperazinium iodide (a nicotinic agonist) evokes noradrenaline release from human neuroblastoma SH-SY5Y cells that have been pretreated with 12-O-tetradecanoylphorbol 13-acetate for 8 min. This effect of dimethylphenylpiperazinium iodide was inhibited by 1 microM mecamylamine but not by 1 microM atropine, which suggests that SH-SY5Y cells express nicotinic receptors coupled to the release of noradrenaline. Dimethylphenylpiperazinium iodide-evoked release was enhanced by 5 microM Bay K 8644 (an L-type calcium agonist) and inhibited by 1 microM nifedipine. Dimethylphenylpiperazinium iodide depolarised SH-SY5Y cells and enhanced the level of intracellular calcium in cells loaded with fura 2. The effects of dimethylphenylpiperazinium iodide on noradrenaline release, depolarisation, and intracellular calcium levels were all inhibited by 1 microM desmethylimipramine. The results of this study show that nicotinic receptors in SH-SY5Y cells stimulate noradrenaline release by activation of L-type calcium channels.

摘要

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