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本文引用的文献

1
Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.用于细胞生长和存活的快速比色测定法。对四氮唑染料程序的改进提高了灵敏度和可靠性。
J Immunol Methods. 1986 May 22;89(2):271-7. doi: 10.1016/0022-1759(86)90368-6.
2
Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases.2'-O-甲基寡核糖核苷酸及其四生物素化衍生物的高效化学合成；对RNA或DNA特异性核酸酶降解具有抗性的新型探针。
Nucleic Acids Res. 1989 May 11;17(9):3373-86. doi: 10.1093/nar/17.9.3373.
3
RNA structure prediction.RNA结构预测
Annu Rev Biophys Biophys Chem. 1988;17:167-92. doi: 10.1146/annurev.bb.17.060188.001123.
4
Nucleoside phosphorothioates.硫代磷酸核苷
Annu Rev Biochem. 1985;54:367-402. doi: 10.1146/annurev.bi.54.070185.002055.
5
Direct measurement of oligonucleotide substrate binding to wild-type and mutant ribozymes from Tetrahymena.直接测量寡核苷酸底物与来自嗜热四膜虫的野生型和突变型核酶的结合。
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8187-91. doi: 10.1073/pnas.87.21.8187.
6
Inhibition of HIV-LTR gene expression by oligonucleotides targeted to the TAR element.靶向TAR元件的寡核苷酸对HIV-LTR基因表达的抑制作用。
Nucleic Acids Res. 1991 Jun 25;19(12):3359-68. doi: 10.1093/nar/19.12.3359.
7
The rapid identification of HIV protease inhibitors through the synthesis and screening of defined peptide mixtures.通过合成和筛选特定肽混合物快速鉴定HIV蛋白酶抑制剂。
Biochem Biophys Res Commun. 1991 Nov 27;181(1):402-8. doi: 10.1016/s0006-291x(05)81433-0.
8
Medicinal chemistry of antisense oligonucleotides--future opportunities.反义寡核苷酸的药物化学——未来机遇
Anticancer Drug Des. 1991 Dec;6(6):585-607.
9
Selection of single-stranded DNA molecules that bind and inhibit human thrombin.结合并抑制人凝血酶的单链DNA分子的筛选。
Nature. 1992 Feb 6;355(6360):564-6. doi: 10.1038/355564a0.
10
Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery.用于基础研究和药物发现的合成肽组合文库的生成与应用。
Nature. 1991 Nov 7;354(6348):84-6. doi: 10.1038/354084a0.

用于药物发现的寡核苷酸组合文库的合理筛选。

Rational screening of oligonucleotide combinatorial libraries for drug discovery.

作者信息

Ecker D J, Vickers T A, Hanecak R, Driver V, Anderson K

机构信息

ISIS Pharmaceuticals, Carlsbad, CA 92008.

出版信息

Nucleic Acids Res. 1993 Apr 25;21(8):1853-6. doi: 10.1093/nar/21.8.1853.

DOI:10.1093/nar/21.8.1853
PMID:7684128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309424/
Abstract

Combinatorial strategies offer the potential to generate and screen extremely large numbers of compounds and to identify individual molecules with a desired binding specificity or pharmacological activity. We describe a combinatorial strategy for oligonucleotides in which the library is generated and screened without using enzymes. Freedom from enzymes enables the use of oligonucleotide analogues. This dramatically extends the scope of both the compounds and the targets that may be screened. We demonstrate the utility of the method by screening 2'-O-Methyl and phosphorothioate oligonucleotide analogue libraries. Compounds have been identified that bind to the activated H-ras mRNA and that have potent antiviral activity against the human herpes simplex virus.

摘要

组合策略提供了生成和筛选大量化合物以及鉴定具有所需结合特异性或药理活性的单个分子的潜力。我们描述了一种用于寡核苷酸的组合策略，其中文库的生成和筛选不使用酶。无需酶使得能够使用寡核苷酸类似物。这极大地扩展了可筛选的化合物和靶标的范围。我们通过筛选2'-O-甲基和硫代磷酸酯寡核苷酸类似文库证明了该方法的实用性。已鉴定出与活化的H-ras mRNA结合且对人单纯疱疹病毒具有强效抗病毒活性的化合物。