Zoja C, Rambaldi A, Remuzzi G
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Ren Physiol Biochem. 1993 Jan-Apr;16(1-2):89-92. doi: 10.1159/000173754.
Our previous in vitro study demonstrated that interleukin-1 (IL-1) stimulates gene expression and production of leukocyte chemotactic factors, colony-stimulating factors and interleukin-6 in human mesangial cells in culture. Here we investigated whether IL-1 regulates its own gene expression in human mesangial cells. Northern blot experiments showed that IL-1 induced IL-1 mRNA expression in a dose-dependent manner. The action of IL-1 on mesangial cells was mediated through the IL-1 receptor type I, which is constitutively expressed by mesangial cells. Recombinant IL-1 receptor antagonist blocked the IL-1 mRNA as well as interleukin-6 and interleukin-8 gene expression induced by IL-1. These data support the crucial role played by IL-1 in regulating mesangial cell cytokine genes within the glomerulus during immunological or inflammatory processes.
我们之前的体外研究表明，白细胞介素-1（IL-1）可刺激培养的人系膜细胞中白细胞趋化因子、集落刺激因子和白细胞介素-6的基因表达及产生。在此，我们研究了IL-1是否在人系膜细胞中调节其自身的基因表达。Northern印迹实验表明，IL-1以剂量依赖的方式诱导IL-1 mRNA表达。IL-1对系膜细胞的作用是通过系膜细胞组成性表达的I型IL-1受体介导的。重组IL-1受体拮抗剂可阻断IL-1诱导的IL-1 mRNA以及白细胞介素-6和白细胞介素-8基因表达。这些数据支持了IL-1在免疫或炎症过程中调节肾小球内系膜细胞细胞因子基因方面所起的关键作用。
